Oct4 plays a role in 2, 3, 7, 8 - tetrachlorobenzo-p-dioxin (TCDD) inducing cleft palate and inhibiting mesenchymal proliferation.
Animals
Apoptosis
Basic Helix-Loop-Helix Transcription Factors
/ genetics
Cell Proliferation
Cleft Palate
/ chemically induced
DNA Methylation
Disease Models, Animal
Female
Male
Mesoderm
/ abnormalities
Mice, Inbred C57BL
Octamer Transcription Factor-3
/ genetics
Palate
/ abnormalities
Polychlorinated Dibenzodioxins
Pregnancy
Promoter Regions, Genetic
Receptors, Aryl Hydrocarbon
/ genetics
Signal Transduction
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)
Cleft palate
Octamer-binding transcription factor 4
Proliferation
Journal
Toxicology
ISSN: 1879-3185
Titre abrégé: Toxicology
Pays: Ireland
ID NLM: 0361055
Informations de publication
Date de publication:
30 05 2020
30 05 2020
Historique:
received:
06
12
2019
revised:
17
03
2020
accepted:
25
03
2020
pubmed:
14
4
2020
medline:
1
9
2020
entrez:
14
4
2020
Statut:
ppublish
Résumé
As a common birth defect, Cleft palate can be caused by the disturbance during the developmental process of the palatal shelves. The 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD) is a well-known environmental teratogenic agent for cleft palate and Aryl hydrocarbon receptor (AhR) pathway can be activated by dioxins. Oct4 as a pluripotent stem cell transcription factor is also involved in the process of embryonic development. The AHR and retinoid receptors have cross-talk at CYP1A1 (cytochrome P450, family 1, subfamily A, polypeptide 1) promoter. There are also bidirectional talk between AhR and Oct4. In this study, we used C57/BL6 N mice and TCDD (64 μg/Kg body weight) to establish a model of fetal cleft palate to observe the effects of dioxin on fetal mesenchymal proliferation and apoptosis, and explore the role of Oct4 in inducing cleft palate. The results showed that dioxin inhibited mesenchymal proliferation and promoted apoptosis. In addition, dioxin inhibited Oct4 expression, and preliminary data suggest that hypermethylation of the Oct4 promoter may be a putative mechanism, suggesting that TCDD might induce cleft palate by inhibiting the proliferation of palatal mesenchymal cells mediated by Oct4.
Identifiants
pubmed: 32283119
pii: S0300-483X(20)30083-4
doi: 10.1016/j.tox.2020.152444
pii:
doi:
Substances chimiques
Ahr protein, mouse
0
Basic Helix-Loop-Helix Transcription Factors
0
Octamer Transcription Factor-3
0
Polychlorinated Dibenzodioxins
0
Pou5f1 protein, mouse
0
Receptors, Aryl Hydrocarbon
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
152444Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.