A Circulating Bioreactor Reprograms Cancer Cells Toward a More Mesenchymal Niche.
bioreactors
circulating tumor cells
epithelial to mesenchymal transition
fluidics
programming
shear force
Journal
Advanced biosystems
ISSN: 2366-7478
Titre abrégé: Adv Biosyst
Pays: Germany
ID NLM: 101711718
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
24
06
2019
revised:
15
10
2019
entrez:
16
4
2020
pubmed:
16
4
2020
medline:
23
1
2021
Statut:
ppublish
Résumé
Cancer is a complex and heterogeneous disease, and cancer cells dynamically interact with the mechanical microenvironment such as hydrostatic pressure, fluid shear, and interstitial flow. These factors play an essential role in cell fate and circulating tumor cell heterogeneity, and can influence the cellular phenotype. In this study, a peristaltic continuous flow reactor is designed and applied to HCT-116 colorectal carcinoma cells to mimic the fluid dynamics of circulation. With this intervention, a CD44/CD24-cell subpopulation emerges, and 100 genes are significantly regulated. The expression of cells at 4 h in the flow reactor is very similar to TGF-ß treatment, which is an inducer of epithelial-mesenchymal transition. ATF3 and SERPINE1 are significantly upregulated in these groups, suggesting that the mesenchymal transition is induced through this signaling pathway. This flow reactor model is satisfactory on its own to reprogram colorectal cancer cells toward a more mesenchymal niche mimicking circulation of the blood.
Identifiants
pubmed: 32293132
doi: 10.1002/adbi.201900139
doi:
Substances chimiques
CD44 protein, human
0
Hyaluronan Receptors
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Pagination
e1900139Informations de copyright
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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