Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD.

Arabidopsis Proteins / genetics Gene Expression Regulation, Plant / genetics NADPH Oxidases / genetics Phosphorylation / genetics Plant Diseases / genetics Plant Immunity / genetics Protein Domains / genetics Protein Serine-Threonine Kinases / genetics Reactive Oxygen Species / metabolism Signal Transduction / genetics Ubiquitination / genetics

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
15 04 2020
Historique:
received: 26 04 2018
accepted: 09 03 2020
entrez: 17 4 2020
pubmed: 17 4 2020
medline: 25 7 2020
Statut: epublish

Résumé

Production of reactive oxygen species (ROS) is critical for successful activation of immune responses against pathogen infection. The plant NADPH oxidase RBOHD is a primary player in ROS production during innate immunity. However, how RBOHD is negatively regulated remains elusive. Here we show that RBOHD is regulated by C-terminal phosphorylation and ubiquitination. Genetic and biochemical analyses reveal that the PBL13 receptor-like cytoplasmic kinase phosphorylates RBOHD's C-terminus and two phosphorylated residues (S862 and T912) affect RBOHD activity and stability, respectively. Using protein array technology, we identified an E3 ubiquitin ligase PIRE (PBL13 interacting RING domain E3 ligase) that interacts with both PBL13 and RBOHD. Mimicking phosphorylation of RBOHD (T912D) results in enhanced ubiquitination and decreased protein abundance. PIRE and PBL13 mutants display higher RBOHD protein accumulation, increased ROS production, and are more resistant to bacterial infection. Thus, our study reveals an intricate post-translational network that negatively regulates the abundance of a conserved NADPH oxidase.

Identifiants

DOI: 10.1038/s41467-020-15601-5 PMID: 32296066 PMC: PMC7160206
pubmed: 32296066
doi: 10.1038/s41467-020-15601-5
pii: 10.1038/s41467-020-15601-5
pmc: PMC7160206
doi:

Substances chimiques

Arabidopsis Proteins 0
Reactive Oxygen Species 0
respiratory burst oxidase homolog D, Arabidopsis EC 1.6.-
NADPH Oxidases EC 1.6.3.-
PBL13 protein, Arabidopsis EC 2.7.11.1
Protein Serine-Threonine Kinases EC 2.7.11.1

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

1838

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM092772
Pays : United States
Organisme : NIGMS NIH HHS
ID : R25 GM056765
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM136402
Pays : United States
Organisme : NIH HHS
ID : S10 OD021801
Pays : United States

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Auteurs

DongHyuk Lee (D)

Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.

Neeraj K Lal (NK)

Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.

Zuh-Jyh Daniel Lin (ZD)

Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.
Donald Danforth Plant Science Center, St Louis, MO, 63132, USA.

Shisong Ma (S)

Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.
School of Life Sciences, University of Science and Technology of China, 230027, Hefei, China.

Jun Liu (J)

Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.
Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China.

Bardo Castro (B)

Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.

Tania Toruño (T)

Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.
ORCID: 0000-0002-9220-016X

Savithramma P Dinesh-Kumar (SP)

Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.

Gitta Coaker (G)

Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA. glcoaker@ucdavis.edu.
ORCID: 0000-0003-0899-2449

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › Regulation of reactive oxygen species during...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › Regulation of reactive oxygen species during...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › NADPH oxidase › Regulation of reactive oxygen species during...
questionsmedicales.fr › Métabolisme › Phosphorylation › Regulation of reactive oxygen species during...
questionsmedicales.fr › Phénomènes physiologiques des plantes › Maladies des plantes › Regulation of reactive oxygen species during...
questionsmedicales.fr › Phénomènes physiologiques des plantes › Immunité des plantes › Regulation of reactive oxygen species during...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › Regulation of reactive oxygen species during...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Regulation of reactive oxygen species during...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Espèces réactives de l'oxygène › Regulation of reactive oxygen species during...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Regulation of reactive oxygen species during...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Modification traductionnelle des protéines › Maturation post-traductionnelle des protéines › Ubiquitination › Regulation of reactive oxygen species during...