The complete genome of lytic Salmonella phage vB_SenM-PA13076 and therapeutic potency in the treatment of lethal Salmonella Enteritidis infections in mice.

Animals Anti-Bacterial Agents / isolation & purification Bacteremia / virology Chickens / virology Feces / virology Genome, Viral Intestines / microbiology Kidney / microbiology Liver / microbiology Mice Myoviridae / genetics Phage Therapy Salmonella Infections / therapy Salmonella Infections, Animal / therapy Salmonella Phages / genetics Salmonella enteritidis / pathogenicity Spleen / microbiology
Genome Infection control Lytic phage Phage therapy Potency Salmonella Enteritidis

Journal

Microbiological research
ISSN: 1618-0623
Titre abrégé: Microbiol Res
Pays: Germany
ID NLM: 9437794

Informations de publication

Date de publication:
Aug 2020
Historique:
received: 01 11 2019
revised: 20 02 2020
accepted: 18 03 2020
pubmed: 17 4 2020
medline: 22 1 2021
entrez: 17 4 2020
Statut: ppublish

Résumé

S. Enteritidis continues to be the most common pathogen of farm animals and a major public health burden worldwide. Using bacteriophages is a potential alternative to antibiotics against S. Enteritidis infection. In this study, the genome analysis of the lytic phage vB_SenM-PA13076 (PA13076) infecting S. Enteritidis revealed a linear, double-stranded DNA genome, which comprised of 52,474 bp and contained 69 ORFs. It belongs to the order Caudovirales; family Myoviridae, genus unclassified. The genes coded for DNA packaging, phage structural proteins, lysis components, DNA recombination, regulation, modification, and replication. No bacterial virulence or drug-resistance genes were detected. The phage PA13076 protected mice from a lethal dose of S. Enteritidis 13076

Identifiants

DOI: 10.1016/j.micres.2020.126471 PMID: 32298944
pubmed: 32298944
pii: S0944-5013(19)31226-1
doi: 10.1016/j.micres.2020.126471
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

126471

Informations de copyright

Copyright © 2020 Elsevier GmbH. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no conflict of interest.

Auteurs

Hongduo Bao (H)

Institute of Food Safety and Nutrition, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China; College of Forestry, Nanjing Forestry University, Nanjing, 210037, China; Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban, 4000, South Africa; Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Pietermaritzburg Campus, Private Bag X01, Pietermaritzburg, 3201, South Africa.

Yan Zhou (Y)

Institute of Food Safety and Nutrition, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.

Khashayar Shahin (K)

Institute of Food Safety and Nutrition, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.

Hui Zhang (H)

Institute of Food Safety and Nutrition, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.

Fuliang Cao (F)

College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.

Maoda Pang (M)

Institute of Food Safety and Nutrition, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.

Xuhui Zhang (X)

College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.

Shujiao Zhu (S)

Institute of Food Safety and Nutrition, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.

Ademola Olaniran (A)

Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban, 4000, South Africa.

Stefan Schmidt (S)

Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Pietermaritzburg Campus, Private Bag X01, Pietermaritzburg, 3201, South Africa. Electronic address: Schmidts@ukzn.ac.za.

Ran Wang (R)

Institute of Food Safety and Nutrition, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. Electronic address: ranwang@jaas.ac.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Inflammation › Sepsie › Bactériémie › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Oiseaux › Poulets › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Liquides et sécrétions biologiques › Fèces › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome viral › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Système digestif › Tube digestif › Intestins › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Appareil urogénital › Voies urinaires › Rein › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Système digestif › Foie › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Virus › Virus à ADN › Caudovirales › Myoviridae › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Thérapeutique › Biothérapie › Phagothérapie › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Enterobacteriaceae › Salmonelloses › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Maladies de l'animal › Salmonelloses animales › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Virus › Bactériophages › Phages de Salmonella › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Salmonella › Salmonella enterica › Salmonella enteritidis › The complete genome of lytic Salmonella phage...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Système lymphatique › Tissu lymphoïde › Rate › The complete genome of lytic Salmonella phage...