The Ccr4-Not complex monitors the translating ribosome for codon optimality.
Codon
Cryoelectron Microscopy
Multiprotein Complexes
/ chemistry
Peptide Chain Elongation, Translational
Peptide Initiation Factors
/ metabolism
Protein Conformation, alpha-Helical
RNA Stability
RNA, Messenger
/ genetics
RNA-Binding Proteins
/ metabolism
Repressor Proteins
/ chemistry
Ribonucleases
/ chemistry
Ribosomes
/ metabolism
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ chemistry
Transcription Factors
/ chemistry
Ubiquitin-Protein Ligases
/ chemistry
Ubiquitination
Eukaryotic Translation Initiation Factor 5A
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
17 04 2020
17 04 2020
Historique:
received:
10
07
2019
revised:
30
01
2020
accepted:
05
03
2020
entrez:
18
4
2020
pubmed:
18
4
2020
medline:
29
4
2020
Statut:
ppublish
Résumé
Control of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA decay through deadenylation and activation of decapping. We used a combination of cryo-electron microscopy, ribosome profiling, and mRNA stability assays to examine the recruitment of Ccr4-Not to the ribosome via specific interaction of the Not5 subunit with the ribosomal E-site in
Identifiants
pubmed: 32299921
pii: 368/6488/eaay6912
doi: 10.1126/science.aay6912
pmc: PMC8663607
mid: NIHMS1758041
pii:
doi:
Substances chimiques
Codon
0
Multiprotein Complexes
0
NOT5 protein, S cerevisiae
0
Peptide Initiation Factors
0
RNA, Messenger
0
RNA-Binding Proteins
0
Repressor Proteins
0
Saccharomyces cerevisiae Proteins
0
Transcription Factors
0
MOT2 protein, S cerevisiae
EC 2.3.2.27
Ubiquitin-Protein Ligases
EC 2.3.2.27
CCR4 protein, S cerevisiae
EC 3.1.-
Ribonucleases
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM118018
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM125086
Pays : United States
Organisme : NIH HHS
ID : S10 OD018174
Pays : United States
Organisme : Deutsche Forschungsgemeinschaft
Pays : International
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
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