Periprotein lipidomes of
Cell Membrane
/ metabolism
Lipidomics
/ methods
Membrane Lipids
/ metabolism
Membrane Microdomains
/ metabolism
Membrane Proteins
/ metabolism
Phospholipids
/ metabolism
Saccharomyces cerevisiae
/ metabolism
Saccharomyces cerevisiae Proteins
/ metabolism
Sphingolipids
/ metabolism
Sterols
/ metabolism
S. cerevisiae
Saccharomyces cerevisiae
biochemistry
chemical biology
eisosome
lipidomics
membrane domains
membrane proteins
plasma membrane
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
17 04 2020
17 04 2020
Historique:
received:
17
03
2020
accepted:
09
04
2020
pubmed:
18
4
2020
medline:
10
4
2021
entrez:
18
4
2020
Statut:
epublish
Résumé
Yeast tolerates a low pH and high solvent concentrations. The permeability of the plasma membrane (PM) for small molecules is low and lateral diffusion of proteins is slow. These findings suggest a high degree of lipid order, which raises the question of how membrane proteins function in such an environment. The yeast PM is segregated into the Micro-Compartment-of-Can1 (MCC) and Pma1 (MCP), which have different lipid compositions. We extracted proteins from these microdomains via stoichiometric capture of lipids and proteins in styrene-maleic-acid-lipid-particles (SMALPs). We purified SMALP-lipid-protein complexes by chromatography and quantitatively analyzed periprotein lipids located within the diameter defined by one SMALP. Phospholipid and sterol concentrations are similar for MCC and MCP, but sphingolipids are enriched in MCP. Ergosterol is depleted from this periprotein lipidome, whereas phosphatidylserine is enriched relative to the bulk of the plasma membrane. Direct detection of PM lipids in the 'periprotein space' supports the conclusion that proteins function in the presence of a locally disordered lipid state.
Identifiants
pubmed: 32301705
doi: 10.7554/eLife.57003
pii: 57003
pmc: PMC7182430
doi:
pii:
Substances chimiques
Membrane Lipids
0
Membrane Proteins
0
Phospholipids
0
Saccharomyces cerevisiae Proteins
0
Sphingolipids
0
Sterols
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR048632
Pays : United States
Organisme : NIH HHS
ID : AR048632
Pays : United States
Organisme : European Research Council
ID : 670578
Pays : International
Déclaration de conflit d'intérêts
Jv, TC, HS, AJ, BM, BP No competing interests declared
Références
Sci Rep. 2016 Aug 23;6:31443
pubmed: 27550794
Elife. 2016 Jun 09;5:
pubmed: 27278775
Biophys J. 2020 Jan 21;118(2):422-434
pubmed: 31843263
Curr Genet. 1989 Dec;16(5-6):339-46
pubmed: 2692852
J Bacteriol. 1991 Mar;173(6):2026-34
pubmed: 2002005
J Bacteriol. 1941 Nov;42(5):561-74
pubmed: 16560468
Mol Biol Cell. 2003 Nov;14(11):4427-36
pubmed: 14551254
J Cell Biol. 2008 Dec 15;183(6):1075-88
pubmed: 19064668
Yeast. 1999 Oct;15(14):1555-64
pubmed: 10514572
Mol Cell Proteomics. 2008 Feb;7(2):282-9
pubmed: 17951627
Yeast. 1995 May;11(6):493-536
pubmed: 7645343
Cold Spring Harb Perspect Biol. 2011 May 01;3(5):
pubmed: 21454248
Front Cell Dev Biol. 2016 Sep 29;4:106
pubmed: 27747212
Yeast. 2014 Aug;31(8):309-21
pubmed: 24962688
Can J Biochem Physiol. 1959 Aug;37(8):911-7
pubmed: 13671378
Biochemistry. 2015 Nov 17;54(45):6852-61
pubmed: 26497753
J Biol Chem. 2014 Mar 7;289(10):7232-46
pubmed: 24448798
Biochem Biophys Res Commun. 2004 Jun 11;318(4):920-6
pubmed: 15147960
J Cell Biol. 1999 Aug 23;146(4):741-54
pubmed: 10459010
Chem Biol. 2011 Dec 23;18(12):1537-49
pubmed: 22195556
Anal Chem. 2006 Feb 1;78(3):779-87
pubmed: 16448051
Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):3935-40
pubmed: 21368142
J Membr Biol. 1993 Jan;131(2):115-27
pubmed: 8441175
Mol Biol Cell. 2017 Oct 15;28(21):2819-2832
pubmed: 28814503
Nat Cell Biol. 2012 Apr 29;14(6):640-8
pubmed: 22544065
J Cell Sci. 2004 Dec 1;117(Pt 25):6031-41
pubmed: 15536122
Nature. 2003 Oct 16;425(6959):737-41
pubmed: 14562106
Traffic. 2018 Mar;19(3):198-214
pubmed: 29282820
Nature. 2006 Feb 23;439(7079):998-1003
pubmed: 16496001
Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19335-40
pubmed: 22087000
J Am Chem Soc. 2009 Jun 10;131(22):7484-5
pubmed: 19449872
Nat Struct Mol Biol. 2010 Sep;17(9):1088-95
pubmed: 20694006
Nat Protoc. 2016 Jul;11(7):1149-62
pubmed: 27254461
Crit Rev Microbiol. 1986;13(3):219-80
pubmed: 3533426
Nucleic Acids Res. 2012 Jan;40(Database issue):D370-6
pubmed: 21890895
J Food Sci. 2015 Apr;80(4):M800-8
pubmed: 25777552
Eur Biophys J. 2017 Jan;46(1):91-101
pubmed: 27815573
Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):E3145-E3154
pubmed: 29559531
J Cell Biol. 2012 Aug 6;198(3):405-20
pubmed: 22869600
Nature. 2012 Jan 09;481(7382):469-74
pubmed: 22230955
Eur Biophys J. 2016 Jan;45(1):3-21
pubmed: 26639665
Yeast. 1999 May;15(7):601-14
pubmed: 10341423
Mol Cell Biol. 2014 Dec;34(24):4447-63
pubmed: 25266656
Nat Immunol. 2018 Apr;19(4):397-406
pubmed: 29531339
Biophys J. 2015 Jan 20;108(2):279-90
pubmed: 25606677
J Biol Chem. 2011 Feb 18;286(7):5043-54
pubmed: 21127065
Biochim Biophys Acta. 1998 May 28;1371(2):345-50
pubmed: 9630718
EMBO J. 2007 Jan 10;26(1):1-8
pubmed: 17170709
Biochim Biophys Acta. 1991 Aug 26;1067(2):139-44
pubmed: 1878367
Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2136-41
pubmed: 19174513
Nat Commun. 2018 Feb 5;9(1):501
pubmed: 29402931
Nat Immunol. 2015 Mar;16(3):258-66
pubmed: 25642819