Molecularly imprinted cryogel cartridges for the selective recognition of tyrosine.

cryogel cartridges hydrophobic interaction metal-chelate coordination molecular imprinting tyrosine

Journal

Biotechnology progress
ISSN: 1520-6033
Titre abrégé: Biotechnol Prog
Pays: United States
ID NLM: 8506292

Informations de publication

Date de publication:
09 2020
Historique:
received: 16 12 2019
revised: 13 04 2020
accepted: 20 04 2020
pubmed: 25 4 2020
medline: 29 9 2021
entrez: 25 4 2020
Statut: ppublish

Résumé

Molecularly imprinted polymers are used for creating a specific cavity and selective recognition sites for the structure of a target molecule in a polymeric structure. In this study, specific molecularly imprinted cryogel cartridges were synthesized using two distinct functional monomers to compare imprinting efficiency for the selective recognition of Tyrosine (Tyr). Tyr-imprinted cryogel cartridge (MIP1) was prepared using metal-chelate coordination for the imprinting process by free-radical bulk polymerization under frozen conditions, and Tyr-imprinted cryogel cartridge (MIP2) was prepared in the same way using hydrophobic effects for imprinting. After the characterization of the cryogel cartridges was carried out, the optimum adsorption conditions of both were determined according to the different parameters such as flow rate (0.5-2.5 ml/min), pH of the medium (4.0-8.0), initial Tyr concentration (0.1-3.0 mg/ml), and temperature (4-45°C). Selectivity experiments of Tyr-imprinted and non-imprinted cryogel cartridges were carried out by using phenylalanine, tryptophan, and cysteine. Besides, the eluted Tyr from MIP1 and MIP2 cryogel cartridge were applied to FPLC system. Also, the reusability experiments of Tyr-imprinted cryogel cartridges was observed no significant decrease in the adsorption capacity.

Identifiants

pubmed: 32329233
doi: 10.1002/btpr.3006
doi:

Substances chimiques

Amino Acids 0
Cryogels 0
Tyrosine 42HK56048U

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e3006

Informations de copyright

© 2020 American Institute of Chemical Engineers.

Références

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Auteurs

Monireh Bakhshpour (M)

Department of Chemistry, Hacettepe University, Ankara, Turkey.

Ilgım Göktürk (I)

Department of Chemistry, Hacettepe University, Ankara, Turkey.

Nilay Bereli (N)

Department of Chemistry, Hacettepe University, Ankara, Turkey.

Adil Denizli (A)

Department of Chemistry, Hacettepe University, Ankara, Turkey.

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