Phenylalanine stable isotope tracer labeling of cow milk and meat and human experimental applications to study dietary protein-derived amino acid availability.
Amino Acids
/ pharmacokinetics
Animals
Biological Availability
Carbon Isotopes
Cattle
Dietary Proteins
/ pharmacokinetics
Digestion
Female
Gastrointestinal Absorption
Humans
Isotope Labeling
/ methods
Lactation
Meat
/ analysis
Milk
/ chemistry
Phenylalanine
/ pharmacokinetics
Postprandial Period
Whey Proteins
/ pharmacokinetics
Amino acid
Caseinate
Digestion
Meat
Protein hydrolysate
Whey
Journal
Clinical nutrition (Edinburgh, Scotland)
ISSN: 1532-1983
Titre abrégé: Clin Nutr
Pays: England
ID NLM: 8309603
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
21
02
2019
revised:
25
02
2020
accepted:
20
03
2020
pubmed:
27
4
2020
medline:
20
8
2021
entrez:
27
4
2020
Statut:
ppublish
Résumé
Availability of dietary protein-derived amino acids (AA) is an important determinant for their utilization in metabolism and for protein synthesis. Intrinsic labeling of protein is the only method to directly trace availability and utilization. The purpose of the present study was to produce labeled milk and meat proteins and investigate how dietary protein-derived AA availability is affected by the protein-meal matrix. Four lactating cows were infused with L-[ring-d Whey and caseinate acquired label to 15-20 mol percent excess (MPE), and the meat proteins reached 0.41-0.73 MPE. The [d Phenylalanine stable isotope-labeled milk and meat were produced and proved a valuable tool to investigate AA absorption characteristics. Dietary protein in food-matrices showed delayed postprandial plasma AA availability as compared to whey protein alone and meat hydrolysate.
Sections du résumé
BACKGROUND & AIMS
Availability of dietary protein-derived amino acids (AA) is an important determinant for their utilization in metabolism and for protein synthesis. Intrinsic labeling of protein is the only method to directly trace availability and utilization. The purpose of the present study was to produce labeled milk and meat proteins and investigate how dietary protein-derived AA availability is affected by the protein-meal matrix.
METHODS
Four lactating cows were infused with L-[ring-d
RESULTS
Whey and caseinate acquired label to 15-20 mol percent excess (MPE), and the meat proteins reached 0.41-0.73 MPE. The [d
CONCLUSIONS
Phenylalanine stable isotope-labeled milk and meat were produced and proved a valuable tool to investigate AA absorption characteristics. Dietary protein in food-matrices showed delayed postprandial plasma AA availability as compared to whey protein alone and meat hydrolysate.
Identifiants
pubmed: 32334880
pii: S0261-5614(20)30134-5
doi: 10.1016/j.clnu.2020.03.017
pii:
doi:
Substances chimiques
Amino Acids
0
Carbon Isotopes
0
Dietary Proteins
0
Whey Proteins
0
Phenylalanine
47E5O17Y3R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3652-3662Informations de copyright
Copyright © 2020 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
Déclaration de conflit d'intérêts
Conflicts of interest Søren Reitelseder and Lars Holm have received funding from The Danish Dairy Research Foundation, Arla Foods Ingredients Group P/S and DC Ingredients. Peter Lund has received funding from The Danish Dairy Research Foundation. Kristian Raaby Poulsen is employee at Arla Foods Ingredients Group P/S, and Erik T. Hansen is employee at DC Ingredients. Otherwise, the authors declare no conflicts of interest.