S-(4-nitrobenzyl)-6-thioinosine: Tritium labelling at high specific activity.

S-(4-nitrobenzyl)-6-thioinosine nucleoside transporters tritium tritium NMR

Journal

Journal of labelled compounds & radiopharmaceuticals
ISSN: 1099-1344
Titre abrégé: J Labelled Comp Radiopharm
Pays: England
ID NLM: 7610510

Informations de publication

Date de publication:
07 2020
Historique:
received: 24 03 2020
accepted: 21 04 2020
pubmed: 27 4 2020
medline: 25 2 2023
entrez: 27 4 2020
Statut: ppublish

Résumé

The one-step tritiation of S-(4-nitrobenzyl)-6-thioinosine is described with characterization of the product by tritium NMR as well as mass spectrometry. The storage, stability, and repurification of [benzyl methylene-

Identifiants

pubmed: 32335922
doi: 10.1002/jlcr.3844
doi:

Substances chimiques

Tritium 10028-17-8
Thioinosine 46S541971T

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

426-429

Informations de copyright

© 2020 John Wiley & Sons, Ltd.

Références

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Plagemann PGW, Estensen RD. Cytochalasin B VI. Competitive inhibition of nucleoside transport by cultured Novikoff rat hepatoma cells. J Cell Biol. 1972;55(1):179-185.
Paul B, Chen MF, Paterson ARP. Inhibitors of nucleoside transport. A structure-activity study using human erythrocytes. J Med Chem. 1975;18(10):968-973.
Bruns RF, Daly JW, Snyder SH. Adenosine receptor binding: structure-activity analysis generates extremely potent xanthine antagonists. Proc Natl Acad Sci. 1983;80:2077-2080.
Singh AK, Klein RS. Synthesis of tritium-labelled 9-deazainosine. J Label Compd Radiopharm. 1988;25:1219-1228.
Egan JA, Filer CN. Tritiation and characterization of several suicide substrate enzyme inactivators. Appl Radiat Isot. 2014;94:302-305.
Nishio M. Stereoselective deuterium exchange of methylene hydrogens of benzyl p-chlorophenyl sulfoxide. Chem Commun. 1968;562-564.
Wolfe S, Rauk A. The absolute stereochemistry of H-D exchange in benzyl methyl sulfoxide. The origin of the kinetic nonequivalence of the methylene protons. Chem Commun. 1966;778-780.
Rauk A, Buncel E, Moir RY, Wolfe S. Hydrogen exchange in benzyl methyl sulfoxide. Kinetic and spectroscopic nonequivalence of methylene protons. J Am Chem Soc. 1965;87:5498-5500.
Yadav V, Chu CK, Rais RH, et al. Synthesis, biological activity and molecular modeling of 6-benzylthioinosine analogues as subversive substrates of Toxoplasma gondii adenosine kinase. J Med Chem. 2004;47(8):1987-1996.
Saljoughian M, Morimoto H, Williams PG. A general synthesis of very high specific activity tritiomethyl iodide. J C S Perkin Trans 1. 1990;1803-1808.
Sen RP, Delicado EG, Alvarez A, Brocklebank AM, Wiley JS, Miras-Portugal MT. Flow cytometric studies of nucleoside transport regulation in single chromaffin cells. FEBS Lett. 1998;422(3):368-372.
Delicado EG, Sen RP, Miras-Portugal MT. Effects of phorbol esters and secretagogues on nitrobenzylthioinosine binding to nucleoside transporters and nucleoside uptake in cultured chromaffin cells. Biochem J. 1991;279:651-655.

Auteurs

Crist N Filer (CN)

PerkinElmer Health Sciences Inc., Waltham, Massachusetts, USA.

Chang Byon (C)

PerkinElmer Health Sciences Inc., Waltham, Massachusetts, USA.

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Classifications MeSH