Fast Screening of Whole Blood and Tumor Tissue for Bladder Cancer Biomarkers Using Stochastic Needle Sensors.


Journal

Sensors (Basel, Switzerland)
ISSN: 1424-8220
Titre abrégé: Sensors (Basel)
Pays: Switzerland
ID NLM: 101204366

Informations de publication

Date de publication:
24 Apr 2020
Historique:
received: 01 03 2020
revised: 12 04 2020
accepted: 23 04 2020
entrez: 30 4 2020
pubmed: 30 4 2020
medline: 5 2 2021
Statut: epublish

Résumé

Bladder cancer is one of the most common urologic malignancies, which is more frequent in men than in women. The early diagnosis for this type of cancer still remains a challenge, therefore, the development of a fast screening test for whole blood and tumor tissue samples may save lives. Four biomarkers, p53, E-cadherin, bladder tumor antigen (BTA), and hyaluronic acid were considered for the screening tests using stochastic needle sensors. Three stochastic needle sensors, based on graphite powder and modified with three types of chitosan, were designed and characterized for the screening test. The proposed sensors showed low limits of quantification, and high sensitivity and selectivity levels. The recoveries of p53, E-cadherin, BTA, and hyaluronic acid in whole blood samples and tissue samples were higher than 95.00% with a relative standard deviation lower than 1.00%.

Identifiants

pubmed: 32344622
pii: s20082420
doi: 10.3390/s20082420
pmc: PMC7219496
pii:
doi:

Substances chimiques

Antigens, Neoplasm 0
Biomarkers, Tumor 0
Cadherins 0
bladder tumor-associated antigen 0
Hyaluronic Acid 9004-61-9
Chitosan 9012-76-4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Références

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Auteurs

Raluca-Ioana Stefan-van Staden (RI)

Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Street, 060021 Bucharest-6, Romania.

Damaris-Cristina Gheorghe (DC)

Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Street, 060021 Bucharest-6, Romania.

Viorel Jinga (V)

Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 050474 Bucharest, Romania.

Cristian Sorin Sima (CS)

Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 050474 Bucharest, Romania.

Marius Geanta (M)

Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 050474 Bucharest, Romania.

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Classifications MeSH