The downregulated membrane expression of CD18 in CD34
CD18
Hematopoietic stem cells
Integrins
Long-term repopulating cells
Journal
Stem cell research & therapy
ISSN: 1757-6512
Titre abrégé: Stem Cell Res Ther
Pays: England
ID NLM: 101527581
Informations de publication
Date de publication:
28 04 2020
28 04 2020
Historique:
received:
13
11
2018
accepted:
08
04
2020
revised:
18
03
2020
entrez:
30
4
2020
pubmed:
30
4
2020
medline:
22
6
2021
Statut:
epublish
Résumé
CD18 is the common beta subunit of β Here we have analyzed the expression of CD18 in different subsets of human hematopoietic stem and progenitor cells (HSPCs) from cord blood (CB), bone marrow (BM), and mobilized peripheral blood (mPB) samples. CD34 A downregulated membrane expression of CD18 was associated with a primitive hematopoietic stem cells (HSC) phenotype, as well as with a higher content of quiescent cells and multipotent colony-forming cells (CFCs). Although no differences in the short-term repopulating potential of CD18 Overall, our results indicate that the downregulated membrane expression of CD18 characterizes a primitive population of human hematopoietic repopulating cells.
Sections du résumé
BACKGROUND
CD18 is the common beta subunit of β
METHODS
Here we have analyzed the expression of CD18 in different subsets of human hematopoietic stem and progenitor cells (HSPCs) from cord blood (CB), bone marrow (BM), and mobilized peripheral blood (mPB) samples. CD34
RESULTS
A downregulated membrane expression of CD18 was associated with a primitive hematopoietic stem cells (HSC) phenotype, as well as with a higher content of quiescent cells and multipotent colony-forming cells (CFCs). Although no differences in the short-term repopulating potential of CD18
CONCLUSIONS
Overall, our results indicate that the downregulated membrane expression of CD18 characterizes a primitive population of human hematopoietic repopulating cells.
Identifiants
pubmed: 32345365
doi: 10.1186/s13287-020-01672-0
pii: 10.1186/s13287-020-01672-0
pmc: PMC7189462
doi:
Substances chimiques
Antigens, CD34
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
164Références
Biosci Rep. 2012 Jun;32(3):241-69
pubmed: 22458844
J Immunol Methods. 2009 Aug 15;347(1-2):70-8
pubmed: 19567251
Blood. 2015 Apr 23;125(17):2621-9
pubmed: 25762174
Science. 2011 Jul 8;333(6039):218-21
pubmed: 21737740
Blood. 1996 May 15;87(10):4120-8
pubmed: 8639769
Blood. 1992 Jul 15;80(2):429-36
pubmed: 1378320
J Hematother Stem Cell Res. 2001 Dec;10(6):807-14
pubmed: 11798507
Hum Gene Ther. 1994 Mar;5(3):295-305
pubmed: 7517188
Cell. 2002 Sep 20;110(6):673-87
pubmed: 12297042
Stem Cells. 2013 Feb;31(2):360-71
pubmed: 23165626
Cell Stem Cell. 2007 Dec 13;1(6):635-45
pubmed: 18371405
Stem Cell Reports. 2014 Nov 11;3(5):707-15
pubmed: 25418718
Immunology. 2012 Apr;135(4):268-75
pubmed: 22211918
Lab Invest. 2010 Apr;90(4):599-610
pubmed: 20142805
Mol Cell Biol. 2003 Dec;23(24):9349-60
pubmed: 14645544
Hematology. 2000;5(4):295-302
pubmed: 11399626
Exp Hematol. 1995 Dec;23(14):1535-42
pubmed: 8542944
Biorheology. 2003;40(1-3):189-95
pubmed: 12454404