Multiplex Generation, Tracking, and Functional Screening of Substitution Mutants Using a CRISPR/Retron System.

Clustered Regularly Interspaced Short Palindromic Repeats / genetics Escherichia coli / genetics Gene Editing / methods Gene Library Genome, Bacterial Mutation Plasmids / genetics RNA, Guide, Kinetoplastida / metabolism
CRISPR genome editing multiplexing recombineering retron screening

Journal

ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075

Informations de publication

Date de publication:
15 05 2020
Historique:
pubmed: 30 4 2020
medline: 1 5 2021
entrez: 30 4 2020
Statut: ppublish

Résumé

We developed a clustered regularly interspaced short palindromic repeats (CRISPR)/retron system for multiplexed generation of substitution mutations by coutilization of a retron system that continuously expresses donor DNA and a CRISPR/Cas9 cassette that induces cleavage at target genomic loci. Our system efficiently introduces substitution mutation in the

Identifiants

DOI: 10.1021/acssynbio.0c00002 PMID: 32348672
pubmed: 32348672
doi: 10.1021/acssynbio.0c00002
doi:

Substances chimiques

RNA, Guide 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1003-1009

Auteurs

Hyeonseob Lim (H)

Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
ORCID: 0000-0002-9470-4142

Soyeong Jun (S)

Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
ORCID: 0000-0001-8419-7098

Minjeong Park (M)

Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.

Junghak Lim (J)

Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.

Jaehwan Jeong (J)

Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.

Ji Hyun Lee (JH)

Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Department of Biomedical Science and Technology, Kyung Hee Medical Science Research Institute, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
ORCID: 0000-0003-3640-2928

Duhee Bang (D)

Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
ORCID: 0000-0001-8775-9877

Articles similaires

Spatial multiplex analysis of lung cancer reveals that regulatory T cells attenuate KRAS-G12C inhibitor-induced immune responses.

Megan Cole, Panayiotis Anastasiou, Claudia Lee et al.
1.00
T-Lymphocytes, Regulatory Lung Neoplasms Proto-Oncogene Proteins p21(ras) Animals Humans

Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis.

Spencer D Shelton, Sara House, Luiza Martins Nascentes Melo et al.
1.00
DNA, Mitochondrial Humans Melanoma Mutation Neoplasm Metastasis

Prevalence and implications of fragile X premutation screening in Thailand.

Areerat Hnoonual, Sunita Kaewfai, Chanin Limwongse et al.
1.00
Humans Fragile X Mental Retardation Protein Thailand Male Female

More T cell receptors to the RAScue in cancer?

Eric Tran
1.00
Humans Receptors, Antigen, T-Cell Proto-Oncogene Proteins p21(ras) Pancreatic Neoplasms T-Lymphocytes

Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › Multiplex Generation, Tracking, and Functional...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Multiplex Generation, Tracking, and Functional...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Multiplex Generation, Tracking, and Functional...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Banque de gènes › Multiplex Generation, Tracking, and Functional...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome bactérien › Multiplex Generation, Tracking, and Functional...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Multiplex Generation, Tracking, and Functional...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › Multiplex Generation, Tracking, and Functional...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › ARN guide › Multiplex Generation, Tracking, and Functional...