An immuno-dot blot assay for screening histidine kinase inhibitors.
Anti-N3-phosphohistidine antibody
Histidine kinase inhibitor
Immuno-dot blotting
Inhibition profiling
Inhibitor screening
Two-component signal transduction system
Journal
Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535
Informations de publication
Date de publication:
01 07 2020
01 07 2020
Historique:
received:
09
03
2020
revised:
26
04
2020
accepted:
27
04
2020
pubmed:
4
5
2020
medline:
20
1
2021
entrez:
4
5
2020
Statut:
ppublish
Résumé
Two-component signal transduction systems (TCSs), consisting of a histidine kinase (HK) and its cognate response regulator, are ubiquitous among bacteria and are associated with the virulence of pathogens. TCSs are potential targets for alternative antibiotics and antivirulence agents. It is, thus, very important to determine HK activity in bacterial TCSs. Here, we describe an immuno-dot blot assay for the inhibition profiling of HKs using the anti-N3-phosphohistidine antibody. This simple method promises reliable detection of HK activity, and it is likely applicable in high-throughput screening of HK inhibitors.
Identifiants
pubmed: 32360198
pii: S0003-2697(20)30297-9
doi: 10.1016/j.ab.2020.113765
pii:
doi:
Substances chimiques
Protein Kinase Inhibitors
0
Quinones
0
waldiomycin
0
Histidine Kinase
EC 2.7.13.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113765Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.