Time-Resolved Fluorescence Anisotropy of a Molecular Rotor Resolves Microscopic Viscosity Parameters in Complex Environments.
lipid droplets
molecular dynamics simulations
molecular rotors
time-resolved fluorescence anisotropy
viscosity imaging time-correlated single photon counting (TCSPC)
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
06
12
2019
revised:
14
02
2020
accepted:
06
03
2020
pubmed:
5
5
2020
medline:
24
6
2021
entrez:
5
5
2020
Statut:
ppublish
Résumé
Understanding viscosity in complex environments remains a largely unanswered question despite its importance in determining reaction rates in vivo. Here, time-resolved fluorescence anisotropy imaging (TR-FAIM) is combined with fluorescent molecular rotors (FMRs) to simultaneously determine two non-equivalent viscosity-related parameters in complex heterogeneous environments. The parameters, FMR rotational correlation time and lifetime, are extracted from fluorescence anisotropy decays, which in heterogeneous environments show dip-and-rise behavior due to multiple dye populations. Decays of this kind are found both in artificially constructed adiposomes and in live cell lipid droplet organelles. Molecular dynamics simulations are used to assign each population to nano-environments within the lipid systems. The less viscous population corresponds to the state showing an average 25° tilt to the lipid membrane normal, and the more viscous population to the state showing an average 55° tilt. This combined experimental and simulation approach enables a comprehensive description of the FMR probe behavior within viscous nano-environments in complex, biological systems.
Identifiants
pubmed: 32363742
doi: 10.1002/smll.201907139
doi:
Substances chimiques
Fluorescent Dyes
0
Lipids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1907139Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R004803/1
Pays : United Kingdom
Informations de copyright
© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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