Periostin Plays a Key Role in Radioresistance of Head and Neck Cancer Cells
Biomarkers, Tumor
/ metabolism
Cell Adhesion Molecules
/ metabolism
Cell Line, Tumor
Down-Regulation
Epithelial-Mesenchymal Transition
Epithelium
/ metabolism
Gene Expression Regulation, Neoplastic
Head and Neck Neoplasms
/ genetics
Humans
Mesoderm
/ pathology
Neoplasm Invasiveness
Radiation Tolerance
/ genetics
Signal Transduction
/ genetics
Squamous Cell Carcinoma of Head and Neck
/ metabolism
Periostin
epithelial-to-mesenchyma transition
head and neck cancer
radioresistance
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
11
03
2020
revised:
24
03
2020
accepted:
01
04
2020
entrez:
6
5
2020
pubmed:
6
5
2020
medline:
15
5
2020
Statut:
ppublish
Résumé
Head and neck squamous cell carcinoma (HNSCC) is an aggressive head and neck malignancy. The aim of this study was to elucidate the role of periostin (POSTN) in the epithelial-to-mesenchymal transition (EMT) process mediating the acquisition of radioresistance in HNSCC. The expression levels of EMT hallmark genes including POSTN and Erk/Akt signaling pathways were compared between radiosensitive and radioresistant HNSCC cells. POSTN mRNA expression was higher in radioresistant HNSCC cells, and silencing POSTN significantly impaired their invasiveness under the effect of EMT process represented by up-regulation of mesenchymal markers and down-regulation of an epithelial marker. Expression levels of Erk and Akt were higher in radioresistant cells. POSTN in association with the Erk and Akt signaling pathways was up-regulated during the EMT process, leading to the conversion of radiosensitive to radioresistant HNSCC cells. POSTN may be a key marker for predicting the radioresistance and therapeutic target of HNSCC.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
Head and neck squamous cell carcinoma (HNSCC) is an aggressive head and neck malignancy. The aim of this study was to elucidate the role of periostin (POSTN) in the epithelial-to-mesenchymal transition (EMT) process mediating the acquisition of radioresistance in HNSCC.
MATERIALS AND METHODS
METHODS
The expression levels of EMT hallmark genes including POSTN and Erk/Akt signaling pathways were compared between radiosensitive and radioresistant HNSCC cells.
RESULTS
RESULTS
POSTN mRNA expression was higher in radioresistant HNSCC cells, and silencing POSTN significantly impaired their invasiveness under the effect of EMT process represented by up-regulation of mesenchymal markers and down-regulation of an epithelial marker. Expression levels of Erk and Akt were higher in radioresistant cells.
CONCLUSION
CONCLUSIONS
POSTN in association with the Erk and Akt signaling pathways was up-regulated during the EMT process, leading to the conversion of radiosensitive to radioresistant HNSCC cells. POSTN may be a key marker for predicting the radioresistance and therapeutic target of HNSCC.
Identifiants
pubmed: 32366407
pii: 40/5/2627
doi: 10.21873/anticanres.14233
doi:
Substances chimiques
Biomarkers, Tumor
0
Cell Adhesion Molecules
0
POSTN protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2627-2635Informations de copyright
Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.