The piRNA response to BmNPV infection in the silkworm fat body and midgut.
BmNPV
fat body
midgut
piRNA
silkworm
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
revised:
23
04
2020
received:
24
12
2019
accepted:
23
04
2020
pubmed:
6
5
2020
medline:
9
6
2021
entrez:
6
5
2020
Statut:
ppublish
Résumé
Bombyx mori nucleopolyhedrovirus (BmNPV) is a DNA virus that causes huge losses to the silkworm industry but the piRNA responses during BmNPV infection in the silkworm remain uninvestigated. Here, silkworm piRNA profiles of uninfected and BmNPV-infected fat body and midgut were determined by high-through sequencing in the early stages of BmNPV infection. A total of 2675 and 3396 genome-derived piRNAs were identified from fat body and midgut, respectively. These genome-derived piRNAs mainly originated from unannotated instead of transposon regions in the silkworm genome. In total, 572 piRNAs were associated with 280 putative target genes in fat body and 805 piRNAs with 380 target genes in midgut. Compared to uninfected tissues, 322 and 129 piRNAs were significantly upregulated in BmNPV-infected fat body and midgut, respectively. In addition, 276 and 117 piRNAs were significantly downregulated. Moreover, differentially expressed (DE) piRNAs during BmNPV infection differed significantly between fat body and midgut. Putative DE piRNA-targeted genes were associated with "response to stimulus" and "environmental information processing" in fat body after infection with BmNPV, which may indicate an active piRNA response to BmNPV infection in fat body. This study may lay the foundation for future research of the potential roles of the piRNA pathway and specific piRNAs in BmNPV pathogenesis.
Identifiants
pubmed: 32367653
doi: 10.1111/1744-7917.12796
doi:
Substances chimiques
RNA, Small Interfering
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
662-679Subventions
Organisme : Guangdong Provincial Promotion Project on Preservation and Utilization of Local Breed of Livestock and Poultry
ID : 2018-143
Organisme : South China Agricultural University Youth Science and Technology Talents Cultivation Special Fund
Organisme : Natural Science Foundation of Guangdong Province
ID : 2018A030310210
Organisme : National Natural Science Foundation of China
ID : 31872426
Organisme : China Postdoctoral Science Foundation
ID : 2017M622714
Informations de copyright
© 2020 Institute of Zoology, Chinese Academy of Sciences.
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