Esophageal microbiome signature in patients with Barrett's esophagus and esophageal adenocarcinoma.
Adenocarcinoma
/ microbiology
Bacteria
/ classification
Barrett Esophagus
/ microbiology
Case-Control Studies
DNA, Bacterial
/ genetics
DNA, Ribosomal
/ genetics
Esophageal Neoplasms
/ microbiology
Esophagus
/ microbiology
Female
Humans
Male
Microbiota
Middle Aged
Phylogeny
Pilot Projects
RNA, Ribosomal, 16S
/ genetics
Sequence Analysis, DNA
/ methods
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
16
12
2019
accepted:
31
03
2020
entrez:
6
5
2020
pubmed:
6
5
2020
medline:
28
7
2020
Statut:
epublish
Résumé
Preliminary studies suggested a possible correlation of microbiota with Barrett's esophagus (BE) and esophageal adenocarcinoma (EAC), where the need for tools to ameliorate its poor prognosis is mandatory. We explored the potential signature of esophageal microbiota and its predicted functional profile along the continuous spectrum from BE to EAC. We analyzed through 16S-based amplicon sequencing the mucosal microbiota and the microbiota-related functional predictions in 10 BE and 6 EAC patients compared with 10 controls, exploring also potential differences between the metaplastic mucosa (BEM) and the adjacent normal areas of BE patients (BEU). BEM and EAC showed a higher level of α and β-diversity. BEM evidenced a decrease of Streptococcus and an increase of Prevotella, Actinobacillus, Veillonella, and Leptotrichia. EAC displayed a striking reduction of Streptococcus, with an increase of Prevotella, Veillonella and Leptotrichia. LefSe analysis identified Leptotrichia as the main taxa distinguishing EAC. BEM showed a decreased α-diversity compared with BEU and a reduction of Bacteroidetes, Prevotella and Fusobacterium. Functional predictions identified peculiar profiles for each group with a high potential for replication and repair in BEM; an upregulated energy, replication and signaling metabolisms, with the fatty-acids biosynthesis and nitrogen and D-alanine pathways down-regulated in EAC. Our pilot study identifies a unique microbial structure and function profile for BE and EAC, as well as for metaplastic and near-normal areas. It proposes a new concept for BE, which could be intended not only as the histological, but, also, as the microbial closest precursor of EAC. This requires further larger follow-up studies, but opens intriguing horizons towards innovative diagnostic and therapeutic options for EAC.
Identifiants
pubmed: 32369505
doi: 10.1371/journal.pone.0231789
pii: PONE-D-19-34724
pmc: PMC7199943
doi:
Substances chimiques
DNA, Bacterial
0
DNA, Ribosomal
0
RNA, Ribosomal, 16S
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0231789Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Environ Microbiol. 2014 Sep;16(9):2905-14
pubmed: 24112768
Nature. 2006 Dec 14;444(7121):860-7
pubmed: 17167474
Arch Iran Med. 2015 Apr;18(4):244-9
pubmed: 25841946
Clin Cancer Res. 2012 Apr 15;18(8):2138-44
pubmed: 22344232
Cell Host Microbe. 2015 May 13;17(5):592-602
pubmed: 25974301
Am J Gastroenterol. 2005 Jun;100(6):1257-64
pubmed: 15929754
BMC Bioinformatics. 2012 Feb 14;13:31
pubmed: 22333067
Gastroenterology. 2009 Aug;137(2):588-97
pubmed: 19394334
Nature. 2012 Aug 9;488(7410):178-84
pubmed: 22797518
Nat Rev Cancer. 2013 Nov;13(11):759-71
pubmed: 24154716
Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4250-5
pubmed: 15016918
Dig Dis. 2018;36(1):56-65
pubmed: 28683448
J Surg Res. 2009 May 1;153(1):172-80
pubmed: 18533190
Clin Infect Dis. 2007 Jul 1;45(1):29-38
pubmed: 17554697
Nature. 2016 Jul 06;535(7610):47
pubmed: 27383978
Nature. 2017 Jan 12;541(7636):169-175
pubmed: 28052061
Lancet Gastroenterol Hepatol. 2017 Jan;2(1):32-42
pubmed: 28404012
Aliment Pharmacol Ther. 2013 Jun;37(11):1084-92
pubmed: 23600758
Cell. 2016 Apr 7;165(2):276-87
pubmed: 27058662
BMC Cancer. 2019 Apr 29;19(1):399
pubmed: 31035942
Gut. 2018 Jan;67(1):120-127
pubmed: 27742762
Sci Rep. 2016 Aug 03;6:30751
pubmed: 27485780
World J Gastroenterol. 2019 May 14;25(18):2149-2161
pubmed: 31143067
Sci Rep. 2019 Oct 1;9(1):14095
pubmed: 31575935
Nature. 2006 Dec 21;444(7122):1022-3
pubmed: 17183309
Ann Thorac Cardiovasc Surg. 2014;20(2):99-105
pubmed: 24583709
Appl Environ Microbiol. 2007 Aug;73(16):5261-7
pubmed: 17586664
Psychoneuroendocrinology. 2019 Jan;99:206-215
pubmed: 30265917
Science. 2015 Apr 3;348(6230):80-6
pubmed: 25838377
Bioinformatics. 2010 Oct 1;26(19):2460-1
pubmed: 20709691
Science. 2017 Oct 20;358(6361):359-365
pubmed: 29051379
Proc Nutr Soc. 2003 Feb;62(1):67-72
pubmed: 12740060
Curr Opin Gastroenterol. 2017 Nov;33(6):422-427
pubmed: 28877044
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
Oncotarget. 2016 Aug 9;7(32):51320-51334
pubmed: 27259999
Nucleic Acids Res. 2016 Jan 4;44(D1):D457-62
pubmed: 26476454
Gut Microbes. 2012 Jul-Aug;3(4):279-88
pubmed: 22572877
Cancer Res. 2016 Nov 1;76(21):6146-6152
pubmed: 27729325
Ann Gastroenterol Surg. 2017 Jun 07;1(2):99-104
pubmed: 29863142
Gastroenterology. 2014 May;146(6):1489-99
pubmed: 24560869
Ann Coloproctol. 2018 Dec;34(6):280-285
pubmed: 30630301
CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108
pubmed: 15761078
J Clin Microbiol. 2012 Apr;50(4):1228-32
pubmed: 22205794
Genome Biol. 2011 Jun 24;12(6):R60
pubmed: 21702898
Appl Environ Microbiol. 2005 Dec;71(12):8228-35
pubmed: 16332807
J Bacteriol. 2010 Oct;192(19):5002-17
pubmed: 20656903
Gut. 2003 Jan;52(1):79-83
pubmed: 12477765
Gut Pathog. 2014 Jun 25;6:26
pubmed: 24995042
Dis Esophagus. 2008;21(6):529-38
pubmed: 18840137
Nat Biotechnol. 2013 Sep;31(9):814-21
pubmed: 23975157
Eur J Immunol. 2015 Jan;45(1):17-31
pubmed: 25328099
Microorganisms. 2019 Jan 13;7(1):
pubmed: 30642137
Int J Mol Sci. 2017 Feb 07;18(2):
pubmed: 28178201
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
Dig Dis Sci. 2004 Aug;49(7-8):1075-83
pubmed: 15387324
Am J Cancer Res. 2015 Sep 15;5(10):3111-22
pubmed: 26693063
Sci Rep. 2017 Nov 21;7(1):15957
pubmed: 29162924
Trends Mol Med. 2017 Jan;23(1):18-30
pubmed: 27986421
Gut. 2018 Aug;67(8):1454-1463
pubmed: 28988196
Microbiome. 2018 Dec 17;6(1):227
pubmed: 30558669
Nat Rev Gastroenterol Hepatol. 2014 Jan;11(1):19-27
pubmed: 23982683
Anaerobe. 2011 Feb;17(1):1-3
pubmed: 21184838
Anaerobe. 2008 Jun;14(3):131-7
pubmed: 18539056
Br J Cancer. 2016 Feb 2;114(3):237-42
pubmed: 26730578