Low temperature modulates natural peel degreening in lemon fruit independently of endogenous ethylene.
Citrus limon
1-methylcyclopropene
carotenoids
chlorophyll
ethylene
low temperature
peel degreening
phytohormones
transcriptome
Journal
Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906
Informations de publication
Date de publication:
06 08 2020
06 08 2020
Historique:
received:
25
11
2019
accepted:
01
05
2020
pubmed:
7
5
2020
medline:
15
5
2021
entrez:
7
5
2020
Statut:
ppublish
Résumé
Peel degreening is an important aspect of fruit ripening in many citrus fruit, and previous studies have shown that it can be advanced by ethylene treatment or by low-temperature storage. However, the important regulators and pathways involved in natural peel degreening remain largely unknown. To determine how natural peel degreening is regulated in lemon fruit (Citrus limon), we studied transcriptome and physiochemical changes in the flavedo in response to ethylene treatment and low temperatures. Treatment with ethylene induced rapid peel degreening, which was strongly inhibited by the ethylene antagonist, 1-methylcyclopropene (1-MCP). Compared with 25 ºC, moderately low storage temperatures of 5-20 °C also triggered peel degreening. Surprisingly, repeated 1-MCP treatments failed to inhibit the peel degreening induced by low temperature. Transcriptome analysis revealed that low temperature and ethylene independently regulated genes associated with chlorophyll degradation, carotenoid metabolism, photosystem proteins, phytohormone biosynthesis and signalling, and transcription factors. Peel degreening of fruit on trees occurred in association with drops in ambient temperature, and it coincided with the differential expression of low temperature-regulated genes. In contrast, genes that were uniquely regulated by ethylene showed no significant expression changes during on-tree peel degreening. Based on these findings, we hypothesize that low temperature plays a prominent role in regulating natural peel degreening independently of ethylene in citrus fruit.
Identifiants
pubmed: 32374848
pii: 5831195
doi: 10.1093/jxb/eraa206
pmc: PMC7410192
doi:
Substances chimiques
Ethylenes
0
Plant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4778-4796Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.
Références
Sci Rep. 2016 Mar 29;6:23609
pubmed: 27021284
J Integr Plant Biol. 2011 May;53(5):358-74
pubmed: 21348940
Plant Physiol. 1981 Oct;68(4):854-6
pubmed: 16662012
Biotechnol Adv. 2006 Jul-Aug;24(4):389-409
pubmed: 16530376
PLoS Genet. 2015 Jul 28;11(7):e1005399
pubmed: 26218222
J Integr Plant Biol. 2011 May;53(5):375-87
pubmed: 21375689
BMC Bioinformatics. 2008 Dec 29;9:559
pubmed: 19114008
Sci Rep. 2017 Jun 21;7(1):4017
pubmed: 28638069
Plant Physiol Biochem. 2013 Sep;70:433-44
pubmed: 23835361
Nucleic Acids Res. 2017 Jul 3;45(W1):W122-W129
pubmed: 28472432
Front Plant Sci. 2019 Jul 05;10:888
pubmed: 31333713
Hortic Res. 2019 Jun 1;6:74
pubmed: 31231532
Plant Physiol. 1970 Mar;45(3):334-8
pubmed: 16657319
Plant Physiol. 2008 Sep;148(1):108-18
pubmed: 18633118
Plant Sci. 2015 Aug;237:57-68
pubmed: 26089152
Plant J. 1999 Dec;20(6):653-61
pubmed: 10652137
Plant J. 2016 Jun;86(5):403-12
pubmed: 27037684
Plant Cell. 2012 Feb;24(2):507-18
pubmed: 22366162
PLoS One. 2014 Dec 31;9(12):e116056
pubmed: 25551568
J Exp Bot. 2020 Jan 1;71(1):330-343
pubmed: 31557301
J Exp Bot. 2012 May;63(8):2873-93
pubmed: 22323274
BMC Plant Biol. 2010 Dec 15;10:276
pubmed: 21159189
J Agric Food Chem. 2009 Jun 10;57(11):4724-32
pubmed: 19441837
Nat Biotechnol. 2014 Jul;32(7):656-62
pubmed: 24908277
J Exp Bot. 2003 Jun;54(387):1615-25
pubmed: 12730273
Plant J. 2007 Aug;51(3):458-67
pubmed: 17655616
Plant Cell Rep. 2012 Sep;31(9):1667-76
pubmed: 22562781
J Exp Bot. 2012 Jan;63(2):963-71
pubmed: 22058408
J Exp Bot. 2006;57(3):633-43
pubmed: 16396998
Plant Physiol. 2012 Sep;160(1):488-97
pubmed: 22797658
BMC Plant Biol. 2018 Mar 21;18(1):47
pubmed: 29562897
Plant Cell. 1996 Sep;8(9):1613-26
pubmed: 8837512
Plant Physiol. 1973 May;51(5):879-82
pubmed: 16658432
J Exp Bot. 2014 Sep;65(17):4705-22
pubmed: 24994760
Plant Physiol. 2010 May;153(1):294-305
pubmed: 20237022
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
Cell. 1998 Jul 24;94(2):261-71
pubmed: 9695954
Plant Physiol. 2004 Feb;134(2):824-37
pubmed: 14739348
Annu Rev Plant Biol. 2006;57:55-77
pubmed: 16669755
Plant Sci. 2017 Jul;260:8-18
pubmed: 28554478
Plant Cell. 2009 Mar;21(3):767-85
pubmed: 19304936
Stat Appl Genet Mol Biol. 2005;4:Article17
pubmed: 16646834
J Exp Bot. 2014 Aug;65(16):4489-90
pubmed: 25221812
Planta. 2004 Jun;219(2):243-52
pubmed: 15014996
J Agric Food Chem. 2006 Jun 28;54(13):4888-95
pubmed: 16787044
Mol Plant. 2014 Dec;7(12):1776-87
pubmed: 25296857
J Exp Bot. 2012 Oct;63(16):5751-61
pubmed: 22945939
J Exp Bot. 2003 Feb;54(383):727-38
pubmed: 12554716
Plant Cell. 2016 Sep;28(9):2147-2160
pubmed: 27604697
Food Chem. 2019 Nov 30;299:125163
pubmed: 31319344
J Exp Bot. 2006;57(10):2153-64
pubmed: 16714310
J Agric Food Chem. 2007 Mar 21;55(6):2356-68
pubmed: 17300198