The photoperiodic response of hypocotyl elongation involves regulation of CDF1 and CDF5 activity.
Journal
Physiologia plantarum
ISSN: 1399-3054
Titre abrégé: Physiol Plant
Pays: Denmark
ID NLM: 1256322
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
31
01
2020
revised:
23
03
2020
accepted:
03
05
2020
pubmed:
8
5
2020
medline:
25
9
2020
entrez:
8
5
2020
Statut:
ppublish
Résumé
Hypocotyl elongation relies on directional cell expansion, a process under light and circadian clock control. Under short photoperiods (SD), hypocotyl elongation in Arabidopsis thaliana follows a rhythmic pattern, a process in which circadian morning-to-midnight waves of the transcriptional repressors PSEUDO-RESPONSE REGULATORS (PRRs) jointly gate PHYTOCHROME-INTERACTING FACTOR (PIF) activity to dawn. Previously, we described CYCLING DOF FACTOR 5 (CDF5) as a target of this antagonistic PRR/PIF dynamic interplay. Under SD, PIFs induce CDF5 accumulation specifically at dawn, when it promotes the expression of positive cell elongation regulators such as YUCCA8 to induce growth. In contrast to SD, hypocotyl elongation under long days (LD) is largely reduced. Here, we examine whether CDF5 is an actor in this photoperiod specific regulation. We report that transcription of CDF5 is robustly induced in SD compared to LD, in accordance with PIFs accumulating to higher levels in SD, and in contrast to other members of the CDF family, whose expression is mainly clock regulated and have similar waveforms in SD and LD. Notably, when CDF5 was constitutively expressed under LD, CDF5 protein accumulated to levels comparable to SD but was inactive in promoting cell elongation. Similar results were observed for CDF1. Our findings indicate that both CDFs can promote cell elongation specifically in shorter photoperiods, however, their activity in LD is inhibited at the post-translational level. These data not only expand our understanding of the biological role of CDF transcription factors, but also identify a previously unrecognized regulatory layer in the photoperiodic response of hypocotyl elongation.
Substances chimiques
Arabidopsis Proteins
0
Basic Helix-Loop-Helix Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
480-490Subventions
Organisme : Agència de Gestió d'Ajuts Universitaris i de Recerca
ID : 2017SGR-718
Organisme : European Commission
ID : PCIG2012-GA-2012-334052
Organisme : Ministerio de Ciencia e Innovación
ID : BIO2015-68460-P
Organisme : Ministerio de Ciencia e Innovación
ID : BIO2015-70812-ERC
Organisme : Ministerio de Ciencia e Innovación
ID : BIO2015-72161-EXP
Organisme : Ministerio de Ciencia e Innovación
ID : PGC2018-099987-B-I00
Organisme : Ministerio de Ciencia e Innovación
ID : RYC-2011-09220
Organisme : Ministerio de Ciencia e Innovación
ID : SEV-2015-0533
Organisme : School of Biological, Earth and Environmental Sciences, University College Cork
ID : Start up funding
Informations de copyright
© 2020 Scandinavian Plant Physiology Society.
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