Differential expression pattern of the proteome in response to cadmium stress based on proteomics analysis of wheat roots.
Cd stress
Chemical forms
Proteomics analysis
Wheat roots
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
07 May 2020
07 May 2020
Historique:
received:
04
02
2019
accepted:
05
04
2020
entrez:
9
5
2020
pubmed:
10
5
2020
medline:
12
1
2021
Statut:
epublish
Résumé
Heavy metal cadmium (Cd) is a common environmental pollutant in soils, which has an negative impacts on crop growth and development. At present, cadmium has become a major soil and water heavy metal pollutant, which not only causes permanent and irreversible health problems for humans, but also causes a significant reduction in crop yields. This study examined the chemical forms of Cd in the roots of two wheat varieties (M1019 and Xinong20) by continuous extraction and analyzed differences in distribution characteristics of Cd in the root cell wall, cytoplasm, and organelles by elemental content determination and subcellular separation. Furthermore, we conducted proteomics analysis of the roots of the two varieties under Cd pollution using mass spectrometry quantitative proteomics techniques. A total of 11,651 proteins were identified, of which 10,532 proteins contained quantitative information. In addition, the differentially expressed proteins in the two varieties were related to DNA replication and repair, protein metabolism, and the glutathione metabolism pathway. The results of this study improve our understanding of the mechanism of plant responses to Cd stress.
Sections du résumé
BACKGROUND
BACKGROUND
Heavy metal cadmium (Cd) is a common environmental pollutant in soils, which has an negative impacts on crop growth and development. At present, cadmium has become a major soil and water heavy metal pollutant, which not only causes permanent and irreversible health problems for humans, but also causes a significant reduction in crop yields.
RESULTS
RESULTS
This study examined the chemical forms of Cd in the roots of two wheat varieties (M1019 and Xinong20) by continuous extraction and analyzed differences in distribution characteristics of Cd in the root cell wall, cytoplasm, and organelles by elemental content determination and subcellular separation. Furthermore, we conducted proteomics analysis of the roots of the two varieties under Cd pollution using mass spectrometry quantitative proteomics techniques. A total of 11,651 proteins were identified, of which 10,532 proteins contained quantitative information. In addition, the differentially expressed proteins in the two varieties were related to DNA replication and repair, protein metabolism, and the glutathione metabolism pathway.
CONCLUSION
CONCLUSIONS
The results of this study improve our understanding of the mechanism of plant responses to Cd stress.
Identifiants
pubmed: 32380942
doi: 10.1186/s12864-020-6716-8
pii: 10.1186/s12864-020-6716-8
pmc: PMC7203821
doi:
Substances chimiques
Plant Proteins
0
Proteome
0
Soil Pollutants
0
Cadmium
00BH33GNGH
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
343Subventions
Organisme : "948" Project of Ministry of Agriculture
ID : 2015Z39 2016X58
Organisme : National 13th Five Key Research and Development Project of China
ID : 2016YFD0101602
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