Deciphering insights of novel recombinant tmTNFα in cell growth inhibition.
Apoptosis
/ drug effects
Caspases
/ metabolism
Cell Line, Tumor
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
G1 Phase Cell Cycle Checkpoints
/ drug effects
G2 Phase Cell Cycle Checkpoints
/ drug effects
HeLa Cells
Hep G2 Cells
Humans
Membrane Potential, Mitochondrial
/ drug effects
Protein Precursors
/ pharmacology
Recombinant Proteins
/ pharmacology
Tumor Necrosis Factor-alpha
/ metabolism
Tumor Necrosis Factors
/ metabolism
Anti-cell proliferation
Apoptosis
Protein therapy
Recombinant tmTNFα
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
22
02
2020
accepted:
30
04
2020
pubmed:
10
5
2020
medline:
17
2
2021
entrez:
10
5
2020
Statut:
ppublish
Résumé
Soluble TNFα, a member of TNF superfamily attributes dual roles in apoptosis and cell proliferation whereas its precursor transmembrane TNFα (tmTNFα) has potential for tumor reduction without initiating proliferation. In this perspective, we recombinantly expressed functional tmTNFα and explored its potential in cell growth inhibition. While structural characterizations of purified tmTNFα revealed integrity of the protein, cell viability assays demonstrated significant antiproliferative effect on HepG2 (IC
Identifiants
pubmed: 32385773
doi: 10.1007/s11033-020-05488-2
pii: 10.1007/s11033-020-05488-2
doi:
Substances chimiques
Protein Precursors
0
Recombinant Proteins
0
Tumor Necrosis Factor-alpha
0
Tumor Necrosis Factors
0
tumor necrosis factor precursor
94948-60-4
Caspases
EC 3.4.22.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3949-3961Subventions
Organisme : Department of Biotechnology , Ministry of Science and Technology
ID : BT/PR13560/COE/34/44/2015