Inhibitory effect of trisodium citrate on biofilms formed by Klebsiella pneumoniae.

Anti-Bacterial Agents / pharmacology Biofilms Citrates Klebsiella pneumoniae
Biofilm Biofilm formation Biofilm inhibition Klebsiella pneumoniae Trisodium citrate

Journal

Journal of global antimicrobial resistance
ISSN: 2213-7173
Titre abrégé: J Glob Antimicrob Resist
Pays: Netherlands
ID NLM: 101622459

Informations de publication

Date de publication:
09 2020
Historique:
received: 08 10 2019
revised: 02 04 2020
accepted: 03 04 2020
pubmed: 11 5 2020
medline: 24 6 2021
entrez: 11 5 2020
Statut: ppublish

Résumé

Klebsiella pneumoniae is a significant nosocomial pathogen related to ventilator-associated pneumonia owing to biofilm formation. Trisodium citrate (TSC) has antibacterial activity, but there is little research on the effect of TSC on biofilm formed by K. pneumoniae. The aims of this study were to evaluate the inhibitory effect of 4% TSC on K. pneumoniae biofilm formation and to determine the best time of TSC addition for biofilm inhibition. A total of 45 K. pneumoniae strains isolated from tracheal tip and sputum specimens were included. Modified Congo red agar was used to screen for biofilm production. Biofilm-positive strains were cultured for 4 days. TSC (4%) was added either initially or 3 days later. Crystal violet staining was used to quantify biofilm mass by measuring the optical density at 570 nm (OD The OD TSC can inhibit K. pneumoniae biofilm formation and has the best effect when added initially.

Identifiants

DOI: 10.1016/j.jgar.2020.04.025 PMID: 32387258
pubmed: 32387258
pii: S2213-7165(20)30117-X
doi: 10.1016/j.jgar.2020.04.025
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0
Citrates 0
trisodium citrate 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

452-456

Informations de copyright

Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Auteurs

Lijuan Chu (L)

Department of Clinical Laboratory Center, Chongqing Health Center for Women and Children, Chongqing 401147, China. Electronic address: clj85@qq.com.

Xingyan Zhou (X)

Department of Clinical Laboratory Center, Chongqing Health Center for Women and Children, Chongqing 401147, China.

Yan Shen (Y)

Department of Clinical Laboratory Center, Chongqing Health Center for Women and Children, Chongqing 401147, China.

Yu Yu (Y)

Department of Clinical Laboratory Center, Chongqing Health Center for Women and Children, Chongqing 401147, China.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Inhibitory effect of trisodium citrate on...
questionsmedicales.fr › Phénomènes microbiologiques › Biofilms › Inhibitory effect of trisodium citrate on...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Triacides carboxyliques › Citrates › Inhibitory effect of trisodium citrate on...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Klebsiella › Klebsiella pneumoniae › Inhibitory effect of trisodium citrate on...