PDK1 regulates auxin transport and Arabidopsis vascular development through AGC1 kinase PAX.
3-Phosphoinositide-Dependent Protein Kinases
/ metabolism
Arabidopsis
/ growth & development
Arabidopsis Proteins
/ metabolism
CRISPR-Associated Protein 9
CRISPR-Cas Systems
Gene Editing
Indoleacetic Acids
/ metabolism
Phloem
/ growth & development
Phosphorylation
Plant Growth Regulators
/ metabolism
Journal
Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
20
09
2019
accepted:
25
03
2020
pubmed:
13
5
2020
medline:
10
2
2021
entrez:
13
5
2020
Statut:
ppublish
Résumé
The 3-phosphoinositide-dependent protein kinase 1 (PDK1) is a conserved master regulator of AGC kinases in eukaryotic organisms. pdk1 loss of function causes a lethal phenotype in animals and yeasts, but only mild phenotypic defects in Arabidopsis thaliana (Arabidopsis). The Arabidopsis genome contains two PDK1-encoding genes, PDK1 and PDK2. Here, we used clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) to generate true loss-of-function pdk1 alleles, which, when combined with pdk2 alleles, showed severe developmental defects including fused cotyledons, a short primary root, dwarf stature and defects in male fertility. We obtained evidence that PDK1 is responsible for AGC1 kinase PROTEIN KINASE ASSOCIATED WITH BRX (PAX) activation by phosphorylation during vascular development, and that the PDK1 phospholipid-binding Pleckstrin Homology domain is not required for this process. Our data indicate that PDK1 regulates polar auxin transport by activating AGC1 clade kinases, resulting in PIN phosphorylation.
Identifiants
pubmed: 32393878
doi: 10.1038/s41477-020-0650-2
pii: 10.1038/s41477-020-0650-2
doi:
Substances chimiques
Arabidopsis Proteins
0
Indoleacetic Acids
0
Plant Growth Regulators
0
3-Phosphoinositide-Dependent Protein Kinases
EC 2.7.11.1
CRISPR-Associated Protein 9
EC 3.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
544-555Références
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