Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens.
MHC class II
Multiplex PCR
Streptococcus
Superantigens
T-cell proliferation
T-cell receptor
Toxin genes
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
21
5
2020
pubmed:
21
5
2020
medline:
9
3
2021
Statut:
ppublish
Résumé
Conventional PCR techniques are laborious and usually not suited for fast screening of large sample numbers in a clinical or research setting. Using this closed-tube multiplex real-time PCR, the presence of all 11 Streptococcus pyogenes superantigen (SAg) genes can be rapidly and accurately characterized. Identifying whether a strain contains a SAg can be done within 4 h compared to conventional methods which would take 11 times as long. This method provides an excellent diagnostic tool as well as a screening tool to help researchers clarify the role of SAgs in S. pyogenes infections.
Identifiants
pubmed: 32430810
doi: 10.1007/978-1-0716-0467-0_2
doi:
Substances chimiques
Antigens, Bacterial
0
DNA Primers
0
Histocompatibility Antigens Class II
0
Superantigens
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM