The Fifth WS/DGAT Enzyme of the Bacterium Marinobacter aquaeolei VT8.
Marinobacter aquaeolei VT8
Neutral lipids
Seed oil
Storage lipids
Wax ester
Wax synthase/acyl CoA:diacylglycerol O-acyltransferase
Journal
Lipids
ISSN: 1558-9307
Titre abrégé: Lipids
Pays: United States
ID NLM: 0060450
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
23
12
2019
revised:
09
04
2020
accepted:
30
04
2020
pubmed:
21
5
2020
medline:
3
8
2021
entrez:
21
5
2020
Statut:
ppublish
Résumé
Wax esters (WE) belong to the class of neutral lipids. They are formed by an esterification of a fatty alcohol and an activated fatty acid. Dependent on the chain length and desaturation degree of the fatty acid and the fatty alcohol moiety, WE can have diverse physicochemical properties. WE derived from monounsaturated long-chain acyl moieties are of industrial interest due to their very good lubrication properties. Whereas WE were obtained in the past from spermaceti organs of the sperm whale, industrial WE are nowadays mostly produced chemically from fossil fuels. In order to produce WE more sustainably, attempts to produce industrial WE in transgenic plants are steadily increasing. To achieve this, different combinations of WE producing enzymes are expressed in developing Arabidopsis thaliana or Camelina sativa seeds. Here we report the identification and characterization of a fifth wax synthase from the organism Marinobacter aquaeolei VT8, MaWSD5. It belongs to the class of bifunctional wax synthase/acyl-CoA:diacylglycerol O-acyltransferases (WSD). The protein was purified to homogeneity. In vivo and in vitro substrate analyses revealed that MaWSD5 is able to synthesize WE but no triacylglycerols. The protein produces WE from saturated and monounsaturated mid- and long-chain substrates. Arabidopsis thaliana seeds expressing a fatty acid reductase from Marinobacter aquaeolei VT8 and MaWSD5 produce WE. Main WE synthesized are 20:1/18:1 and 20:1/20:1. This makes MaWSD5 a suitable candidate for industrial WE production in planta.
Substances chimiques
Acyl Coenzyme A
0
Esters
0
Glycolipids
0
Waxes
0
Acyltransferases
EC 2.3.-
long-chain-alcohol O-fatty-acyltransferase
EC 2.3.1.75
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
479-494Informations de copyright
© 2020 The Authors. Lipids published by Wiley Periodicals, Inc. on behalf of American Oil Chemists' Society.
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