Promoting the accumulation of scopolamine and hyoscyamine in Hyoscyamus niger L. through EMS based mutagenesis.
Biosynthetic Pathways
Ethyl Methanesulfonate
/ toxicity
Gene Expression Regulation, Plant
Hyoscyamine
/ metabolism
Hyoscyamus
/ drug effects
Methyltransferases
/ genetics
Mixed Function Oxygenases
/ genetics
Mutagenesis
Mutagens
/ toxicity
Mutation
Plants, Genetically Modified
/ drug effects
Scopolamine
/ metabolism
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
29
01
2020
accepted:
21
03
2020
entrez:
22
5
2020
pubmed:
22
5
2020
medline:
28
7
2020
Statut:
epublish
Résumé
The overexploitation of medicinal plants is depleting gene pool at an alarming rate. In this scenario inducing the genetic variability through targeted mutations could be beneficial in generating varieties with increased content of active compounds. The present study aimed to develop a reproducible protocol for in vitro multiplication and mutagenesis of Hyoscyamus niger targeting putrescine N-methyltransferase (PMT) and 6β-hydroxy hyoscyamine (H6H) genes of alkaloid biosynthetic pathway. In vitro raised callus were treated with different concentrations (0.01% - 0.1%) of Ethyl Methane Sulfonate (EMS). Emerging multiple shoots and roots were obtained on the MS media supplemented with cytokinins and auxins. Significant effects on morphological characteristics were observed following exposure to different concentrations of EMS. EMS at a concentration of 0.03% was seen to be effective in enhancing the average shoot and root number from 14.5±0.30 to 22.2 ±0.77 and 7.2±0.12 to 8.8±0.72, respectively. The lethal dose (LD50) dose was calculated at 0.08% EMS. The results depicted that EMS has an intense effect on PMT and H6H gene expression and metabolite accumulation. The transcripts of PMT and H6H were significantly upregulated at 0.03-0.05% EMS compared to control. EMS treated explants showed increased accumulation of scopolamine (0.639 μg/g) and hyoscyamine (0.0344μg/g) compared to untreated.
Identifiants
pubmed: 32437389
doi: 10.1371/journal.pone.0231355
pii: PONE-D-20-02645
pmc: PMC7241962
doi:
Substances chimiques
Mutagens
0
Ethyl Methanesulfonate
9H154DI0UP
Scopolamine
DL48G20X8X
Mixed Function Oxygenases
EC 1.-
Methyltransferases
EC 2.1.1.-
putrescine N-methyltransferase
EC 2.1.1.53
Hyoscyamine
PX44XO846X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0231355Déclaration de conflit d'intérêts
No authors have competing interests.
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