Gene expression trajectories during male and female reproductive development in balsam poplar (Populus balsamifera L.).
Chromatin
/ metabolism
Chromatin Assembly and Disassembly
/ physiology
Flowers
/ embryology
Gene Expression Regulation, Developmental
/ genetics
Gene Expression Regulation, Plant
/ genetics
Genes, Plant
/ genetics
Intracellular Signaling Peptides and Proteins
/ metabolism
MADS Domain Proteins
/ metabolism
Meristem
/ embryology
Populus
/ embryology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 05 2020
21 05 2020
Historique:
received:
24
11
2019
accepted:
24
04
2020
entrez:
23
5
2020
pubmed:
23
5
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Plant reproductive development from the first appearance of reproductively committed axes through to floral maturation requires massive and rapid remarshalling of gene expression. In dioecious species such as poplar this is further complicated by divergent male and female developmental programs. We used seven time points in male and female balsam poplar (Populus balsamifera L.) buds and catkins representing the full annual flowering cycle, to elucidate the effects of time and sex on gene expression during reproductive development. Time (developmental stage) is dominant in patterning gene expression with the effect of sex nested within this. Here, we find (1) evidence for five successive waves of alterations to the chromatin landscape which may be important in setting the overall reproductive trajectory, regardless of sex. (2) Each individual developmental stage is further characterized by marked sex-differential gene expression. (3) Consistent sexually differentiated gene expression regardless of developmental stage reveal candidates for high-level regulators of sex and include the female-specific poplar ARR17 homologue. There is also consistent male-biased expression of the MADS-box genes PISTILLATA and APETALA3. Our work provides insights into expression trajectories shaping reproductive development, its potential underlying mechanisms, and sex-specific translation of the genome information into reproductive structures in balsam poplar.
Identifiants
pubmed: 32439903
doi: 10.1038/s41598-020-64938-w
pii: 10.1038/s41598-020-64938-w
pmc: PMC7242425
doi:
Substances chimiques
Chromatin
0
Intracellular Signaling Peptides and Proteins
0
MADS Domain Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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