HOXB2 and FOXC1 synergistically drive the progression of Wilms tumor.
3' Untranslated Regions
/ genetics
Adult
Base Sequence
Cell Line
Cell Movement
Cell Proliferation
Disease Progression
Female
Forkhead Transcription Factors
/ metabolism
Homeodomain Proteins
/ metabolism
Humans
Male
Protein Binding
Transcription Factors
/ metabolism
Wilms Tumor
/ pathology
Young Adult
FOXC1
HOXB2
Malignant development
Wilms tumor
Journal
Experimental and molecular pathology
ISSN: 1096-0945
Titre abrégé: Exp Mol Pathol
Pays: Netherlands
ID NLM: 0370711
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
05
04
2020
revised:
10
05
2020
accepted:
17
05
2020
pubmed:
24
5
2020
medline:
30
10
2020
entrez:
24
5
2020
Statut:
ppublish
Résumé
To uncover the expression patterns of HOXB2 and FOXC1 in Wilms tumor samples, and their synergistical regulations on the development of Wilms tumor. Expression levels of HOXB2 and FOXC1 in 58 cases of Wilms tumor tissues and paracancerous ones were detected. The influences of HOXB2 and FOXC1 on prognosis in Wilms tumor patients were analyzed. Their regulatory effects on proliferative and migratory abilities in WT-CLS1 and HFWT cells were examined by cell counting kit-8 (CCK-8) and Transwell assay, respectively. The interaction between HOXB2 and FOXC1, and their synergistical regulation on the development of Wilms tumor were finally explored. HOXB2 and FOXC1 were upregulated in Wilms tumor tissues. Higher levels of HOXB2 and FOXC1 indicated higher risks of advanced stage and lymphatic metastasis, as well as worse prognosis in Wilms tumor patients. Knockdown of HOXB2 or FOXC1 weakened proliferative and migratory abilities in WT-CLS1 and HFWT cells, while the opposite trends were observed in those overexpressing HOXB2 or FOXC1. The positive interaction between HOXB2 and FOXC1 was identified, which synergistically drove the malignant development of Wilms tumor. HOXB2 and FOXC1 are upregulated in Wilms tumor samples, and they are closely linked to tumor staging and lymphatic metastasis in Wilms tumor patients. HOXB2 and FOXC1 synergistically drive the malignant development of Wilms tumor by stimulating proliferative and migratory potentials.
Identifiants
pubmed: 32445751
pii: S0014-4800(20)30347-6
doi: 10.1016/j.yexmp.2020.104469
pii:
doi:
Substances chimiques
3' Untranslated Regions
0
FOXC1 protein, human
0
Forkhead Transcription Factors
0
HOXB2 protein, human
0
Homeodomain Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104469Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declared no conflict of interest.