A 2-Tyr-1-carboxylate Mononuclear Iron Center Forms the Active Site of a Paracoccus Dimethylformamidase.

Amidohydrolases / chemistry Catalytic Domain Dimethylformamide / chemistry Molecular Structure Paracoccus / enzymology Tyrosine / chemistry
amide bond hydrolysis bioremediation dimethylforamamidase metalloenzymes protein structure

Journal

Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543

Informations de publication

Date de publication:
21 09 2020
Historique:
received: 12 04 2020
revised: 04 05 2020
pubmed: 27 5 2020
medline: 19 3 2021
entrez: 27 5 2020
Statut: ppublish

Résumé

N,N-dimethyl formamide (DMF) is an extensively used organic solvent but is also a potent pollutant. Certain bacterial species from genera such as Paracoccus, Pseudomonas, and Alcaligenes have evolved to use DMF as a sole carbon and nitrogen source for growth via degradation by a dimethylformamidase (DMFase). We show that DMFase from Paracoccus sp. strain DMF is a halophilic and thermostable enzyme comprising a multimeric complex of the α

Identifiants

DOI: 10.1002/anie.202005332 PMID: 32452120 PMC: PMC7686228
pubmed: 32452120
doi: 10.1002/anie.202005332
pmc: PMC7686228
mid: NIHMS1624783
doi:

Substances chimiques

Tyrosine 42HK56048U
Dimethylformamide 8696NH0Y2X
Amidohydrolases EC 3.5.-
N,N-dimethylformamidase EC 3.5.1.-

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

16961-16966

Subventions

Organisme : Medical Research Council
ID : MC_U105192715
Pays : United Kingdom
Organisme : NCI NIH HHS
ID : P30 CA023168
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002529
Pays : United States

Informations de copyright

© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Références

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Angew Chem Int Ed Engl. 2020 Sep 21;59(39):16961-16966
pubmed: 32452120
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pubmed: 30952664
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Auteurs

Chetan Kumar Arya (CK)

Department of Chemistry, Indian Institute of Technology, Kanpur, India.
Institute for Stem Cell Science and Regenerative Medicine, Bangalore, India.
ORCID: 0000-0003-2182-3535

Swati Yadav (S)

National Center for Biological Sciences-TIFR, GKVK Post, Bangalore, India.

Jonathan Fine (J)

Department of Chemistry, Purdue University, West Lafayette, IN, USA.

Ana Casanal (A)

MRC Laboratory of Molecular Biology, Cambridge, UK.
ORCID: 0000-0002-0334-0591

Gaurav Chopra (G)

Department of Chemistry, Purdue University, West Lafayette, IN, USA.
ORCID: 0000-0003-0942-7898

Gurunath Ramanathan (G)

Department of Chemistry, Indian Institute of Technology, Kanpur, India.
ORCID: 0000-0003-4627-1677

Kutti R Vinothkumar (KR)

National Center for Biological Sciences-TIFR, GKVK Post, Bangalore, India.
ORCID: 0000-0002-6746-5684

Ramaswamy Subramanian (R)

Institute for Stem Cell Science and Regenerative Medicine, Bangalore, India.
Department of Biological Sciences and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
ORCID: 0000-0002-6709-190X

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Amidohydrolases › A 2-Tyr-1-carboxylate Mononuclear Iron Center...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Domaine catalytique › A 2-Tyr-1-carboxylate Mononuclear Iron Center...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Formiates › Formamides › N,N-Diméthyl-formamide › A 2-Tyr-1-carboxylate Mononuclear Iron Center...
questionsmedicales.fr › Phénomènes chimiques › Structure moléculaire › A 2-Tyr-1-carboxylate Mononuclear Iron Center...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Rhodobacteraceae › Paracoccus › A 2-Tyr-1-carboxylate Mononuclear Iron Center...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés cycliques › Acides aminés aromatiques › Tyrosine › A 2-Tyr-1-carboxylate Mononuclear Iron Center...