Growth study under combined effects of temperature, pH and salinity and transcriptome analysis revealed adaptations of Aspergillus terreus NTOU4989 to the extreme conditions at Kueishan Island Hydrothermal Vent Field, Taiwan.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
18
12
2019
accepted:
08
05
2020
entrez:
27
5
2020
pubmed:
27
5
2020
medline:
15
8
2020
Statut:
epublish
Résumé
A high diversity of fungi was discovered on various substrates collected at the marine shallow-water Kueishan Island Hydrothermal Vent Field, Taiwan, using culture and metabarcoding methods but whether these fungi can grow and play an active role in such an extreme environment is unknown. We investigated the combined effects of different salinity, temperature and pH on growth of ten fungi (in the genera Aspergillus, Penicillium, Fodinomyces, Microascus, Trichoderma, Verticillium) isolated from the sediment and the vent crab Xenograpsus testudinatus. The growth responses of the tested fungi could be referred to three groups: (1) wide pH, salinity and temperature ranges, (2) salinity-dependent and temperature-sensitive, and (3) temperature-tolerant. Aspergillus terreus NTOU4989 was the only fungus which showed growth at 45 °C, pH 3 and 30 ‰ salinity, and might be active near the vents. We also carried out a transcriptome analysis to understand the molecular adaptations of A. terreus NTOU4989 under these extreme conditions. Data revealed that stress-related genes were differentially expressed at high temperature (45 °C); for instance, mannitol biosynthetic genes were up-regulated while glutathione S-transferase and amino acid oxidase genes down-regulated in response to high temperature. On the other hand, hydrogen ion transmembrane transport genes and phenylalanine ammonia lyase were up-regulated while pH-response transcription factor was down-regulated at pH 3, a relative acidic environment. However, genes related to salt tolerance, such as glycerol lipid metabolism and mitogen-activated protein kinase, were up-regulated in both conditions, possibly related to maintaining water homeostasis. The results of this study revealed the genetic evidence of adaptation in A. terreus NTOU4989 to changes of environmental conditions.
Identifiants
pubmed: 32453769
doi: 10.1371/journal.pone.0233621
pii: PONE-D-19-35103
pmc: PMC7250430
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0233621Déclaration de conflit d'intérêts
The authors have declared that no financial or non-financial competing interests exist.
Références
Eukaryot Cell. 2014 Sep;13(9):1241-53
pubmed: 25084861
Cell Microbiol. 2017 Apr;19(4):
pubmed: 27706915
BMC Genomics. 2017 Aug 4;18(1):579
pubmed: 28778147
Appl Microbiol Biotechnol. 2018 Jan;102(2):897-906
pubmed: 29101425
Nat Protoc. 2012 Mar 01;7(3):562-78
pubmed: 22383036
BMC Genomics. 2013 Feb 28;14:138
pubmed: 23445374
J Biol Chem. 2002 Sep 20;277(38):35523-31
pubmed: 12063243
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Front Microbiol. 2016 Dec 21;7:2048
pubmed: 28066369
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
Biotechnol Res Int. 2015;2015:132635
pubmed: 26881084
G3 (Bethesda). 2015 Jun 26;5(9):1805-14
pubmed: 26116293
Eukaryot Cell. 2009 Nov;8(11):1616-25
pubmed: 19717745
Bioinformatics. 2004 Jun 12;20(9):1453-4
pubmed: 14871861
Eukaryot Cell. 2012 Oct;11(10):1226-38
pubmed: 22903976
BMC Genomics. 2017 Dec 04;18(1):942
pubmed: 29202712
Microb Ecol. 2005 Oct;50(3):408-17
pubmed: 16328655
Environ Microbiol. 2009 Jun;11(6):1588-600
pubmed: 19239486
Nat Biotechnol. 2015 Mar;33(3):290-5
pubmed: 25690850
PLoS One. 2016 Feb 05;11(2):e0148675
pubmed: 26849440
Biomed Res Int. 2017;2017:4378627
pubmed: 28904958
J Microbiol Methods. 1999 Jul;37(1):97-100
pubmed: 10395469
Microbiology. 2004 Oct;150(Pt 10):3315-26
pubmed: 15470111
F1000Res. 2013 Sep 16;2:188
pubmed: 24555089
Front Microbiol. 2017 Sep 21;8:1789
pubmed: 28983284
BMC Bioinformatics. 2011 Aug 04;12:323
pubmed: 21816040
Appl Environ Microbiol. 1995 Apr;61(4):1323-30
pubmed: 7747954
Mycobiology. 2016 Dec;44(4):237-247
pubmed: 28154481
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2426-8
pubmed: 4506763
BMC Genomics. 2010 Jan 15;11:32
pubmed: 20074381
Biosci Biotechnol Biochem. 2016 Sep;80(9):1667-80
pubmed: 27007956
Res Microbiol. 2015 Nov;166(9):700-9
pubmed: 26226336
Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
pubmed: 9843981
Mol Microbiol. 2011 Mar;79(6):1574-93
pubmed: 21269335
Eukaryot Cell. 2007 Jun;6(6):1053-62
pubmed: 17449658
Int J Genomics. 2017;2017:6923849
pubmed: 28770220
Stud Mycol. 2011 Jun 30;69(1):39-55
pubmed: 21892242
Sci Rep. 2016 Dec 12;6:38747
pubmed: 27941917
Sci Rep. 2017 Nov 16;7(1):15694
pubmed: 29146915
PLoS One. 2012;7(3):e33128
pubmed: 22427966
Front Cell Infect Microbiol. 2017 Dec 19;7:520
pubmed: 29312897
Microbiology. 2013 Jan;159(Pt 1):176-190
pubmed: 23154970
PLoS One. 2014 Oct 03;9(10):e109696
pubmed: 25279954
World J Microbiol Biotechnol. 2014 May;30(5):1661-8
pubmed: 24366816
Clin Microbiol Infect. 2017 Oct;23(10):776.e1-776.e5
pubmed: 28412383
PLoS Pathog. 2011 Jul;7(7):e1002145
pubmed: 21811407
BMC Genomics. 2018 Oct 1;19(1):721
pubmed: 30285612
Appl Environ Microbiol. 2009 Oct;75(20):6415-21
pubmed: 19633124
Eukaryot Cell. 2003 Aug;2(4):690-8
pubmed: 12912888
J Biotechnol. 2013 Aug 10;167(1):56-63
pubmed: 23792099
FEMS Microbiol Ecol. 2010 Jul 1;73(1):121-33
pubmed: 20455940
PLoS One. 2019 Dec 30;14(12):e0226616
pubmed: 31887170