Multispecies RNA tomography reveals regulators of hematopoietic stem cell birth in the embryonic aorta.
Animals
Animals, Genetically Modified
Aorta
/ cytology
Cell Tracking
/ methods
Chick Embryo
Embryo, Mammalian
Embryo, Nonmammalian
Gene Expression Regulation, Developmental
Hematopoiesis
/ genetics
Hematopoietic Stem Cells
/ cytology
Humans
Mice
RNA
/ analysis
Sequence Analysis, RNA
/ methods
Single-Cell Analysis
Species Specificity
Stem Cell Niche
/ genetics
Tomography
/ methods
Zebrafish
/ embryology
Journal
Blood
ISSN: 1528-0020
Titre abrégé: Blood
Pays: United States
ID NLM: 7603509
Informations de publication
Date de publication:
13 08 2020
13 08 2020
Historique:
received:
05
12
2019
accepted:
26
04
2020
pubmed:
28
5
2020
medline:
18
3
2021
entrez:
28
5
2020
Statut:
ppublish
Résumé
The defined location of a stem cell within a niche regulates its fate, behavior, and molecular identity via a complex extrinsic regulation that is far from being fully elucidated. To explore the molecular characteristics and key components of the aortic microenvironment, where the first hematopoietic stem cells are generated during development, we performed genome-wide RNA tomography sequencing on zebrafish, chicken, mouse, and human embryos. The resulting anterior-posterior and dorsal-ventral transcriptional maps provided a powerful resource for exploring genes and regulatory pathways active in the aortic microenvironment. By performing interspecies comparative RNA sequencing analyses and functional assays, we explored the complexity of the aortic microenvironment landscape and the fine-tuning of various factors interacting to control hematopoietic stem cell generation, both in time and space in vivo, including the ligand-receptor couple ADM-RAMP2 and SVEP1. Understanding the regulatory function of the local environment will pave the way for improved stem cell production in vitro and clinical cell therapy.
Identifiants
pubmed: 32457985
pii: S0006-4971(20)61798-0
doi: 10.1182/blood.2019004446
pmc: PMC7515669
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
831-844Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 by The American Society of Hematology.
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