FRET kinase sensor development reveals SnRK2/OST1 activation by ABA but not by MeJA and high CO
Abscisic Acid
/ metabolism
Acetates
/ metabolism
Arabidopsis
/ genetics
Arabidopsis Proteins
/ chemistry
Biosensing Techniques
/ methods
Carbon Dioxide
/ metabolism
Cyclopentanes
/ metabolism
Oxylipins
/ metabolism
Plant Proteins
/ genetics
Plant Stomata
/ metabolism
Protein Kinases
/ chemistry
Protein Serine-Threonine Kinases
/ genetics
Recombinant Fusion Proteins
/ genetics
Stress, Physiological
/ genetics
Nicotiana
/ genetics
14-3-3 protein
A. thaliana
SnRK2 OST1 protein kinase
abscisic acid
in vivo FRET imaging
plant biology
plant stress signaling
stomatal guard cells
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
28 05 2020
28 05 2020
Historique:
received:
25
02
2020
accepted:
20
05
2020
pubmed:
29
5
2020
medline:
24
2
2021
entrez:
29
5
2020
Statut:
epublish
Résumé
Sucrose-non-fermenting-1-related protein kinase-2s (SnRK2s) are critical for plant abiotic stress responses, including abscisic acid (ABA) signaling. Here, we develop a genetically encoded reporter for SnRK2 kinase activity. This sensor, named SNACS, shows an increase in the ratio of yellow to cyan fluorescence emission by OST1/SnRK2.6-mediated phosphorylation of a defined serine residue in SNACS. ABA rapidly increases FRET efficiency in
Identifiants
pubmed: 32463362
doi: 10.7554/eLife.56351
pii: 56351
pmc: PMC7289597
doi:
pii:
Substances chimiques
Acetates
0
Arabidopsis Proteins
0
Cyclopentanes
0
Oxylipins
0
Plant Proteins
0
Recombinant Fusion Proteins
0
Carbon Dioxide
142M471B3J
Abscisic Acid
72S9A8J5GW
methyl jasmonate
900N171A0F
Protein Kinases
EC 2.7.-
OST1 protein, Arabidopsis
EC 2.7.1.-
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : GM060396
Pays : United States
Organisme : Eesti Teadusagentuur
ID : PRG719
Pays : International
Organisme : Eesti Teadusagentuur
ID : PUT1133
Pays : International
Organisme : NIGMS NIH HHS
ID : R01 GM060396
Pays : United States
Organisme : National Science Foundation
ID : MCB-1900567
Pays : International
Organisme : National Science Foundation
ID : MCB-1137950
Pays : International
Organisme : Eesti Teadusagentuur
ID : PRG433
Pays : International
Informations de copyright
© 2020, Zhang et al.
Déclaration de conflit d'intérêts
LZ, YT, PH, HK, EM, PK, JS No competing interests declared
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