A tps1Δ persister-like state in Saccharomyces cerevisiae is regulated by MKT1.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
18
03
2020
accepted:
12
05
2020
entrez:
30
5
2020
pubmed:
30
5
2020
medline:
10
9
2020
Statut:
epublish
Résumé
Trehalose metabolism in yeast has been linked to a variety of phenotypes, including heat resistance, desiccation tolerance, carbon-source utilization, and sporulation. The relationships among the several phenotypes of mutants unable to synthesize trehalose are not understood, even though the pathway is highly conserved. One of these phenotypes is that tps1Δ strains cannot reportedly grow on media containing glucose or fructose, even when another carbon source they can use (e.g. galactose) is present. Here we corroborate the recent observation that a small fraction of yeast tps1Δ cells do grow on glucose, unlike the majority of the population. This is not due to a genetic alteration, but instead resembles the persister phenotype documented in many microorganisms and cancer cells undergoing lethal stress. We extend these observations to show that this phenomenon is glucose-specific, as it does not occur on another highly fermented carbon source, fructose. We further demonstrate that this phenomenon appears to be related to mitochondrial complex III function, but unrelated to inorganic phosphate levels in the cell, as had previously been suggested. Finally, we found that this phenomenon is specific to S288C-derived strains, and is the consequence of a variant in the MKT1 gene.
Identifiants
pubmed: 32470059
doi: 10.1371/journal.pone.0233779
pii: PONE-D-20-07801
pmc: PMC7259636
doi:
Substances chimiques
MKT1 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
Fructose
30237-26-4
Trehalose
B8WCK70T7I
Glucosyltransferases
EC 2.4.1.-
trehalose-6-phosphate synthase
EC 2.4.1.15
Electron Transport Complex III
EC 7.1.1.8
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0233779Déclaration de conflit d'intérêts
The authors have read the journal’s policy and have the following conflicts: authors PAG, DGH, RSM, and DB are affiliated with Calico Life Sciences LLC. This does not alter our adherence to all the PLOS ONE policies on sharing data and materials.
Références
Genetics. 2009 Nov;183(3):1141-51
pubmed: 19720856
Bioinformatics. 2004 Nov 22;20(17):3246-8
pubmed: 15180930
Genetics. 2009 Sep;183(1):365-83
pubmed: 19581448
Mol Gen Genet. 1977 Jul 7;154(1):61-6
pubmed: 197389
PLoS Pathog. 2011 Jul;7(7):e1002121
pubmed: 21829345
PLoS Biol. 2003 Nov;1(2):E28
pubmed: 14624238
Biochemistry. 1979 Oct 16;18(21):4487-99
pubmed: 40590
Curr Genet. 2015 Aug;61(3):263-74
pubmed: 25209979
PLoS Genet. 2009 Dec;5(12):e1000760
pubmed: 20011100
Nature. 2010 Apr 15;464(7291):1039-42
pubmed: 20393561
Microbiol Mol Biol Rev. 1999 Sep;63(3):554-69
pubmed: 10477308
Mol Microbiol. 1996 Jun;20(5):981-91
pubmed: 8809751
BMC Evol Biol. 2006 Dec 19;6:109
pubmed: 17178000
Anal Chem. 2010 Dec 1;82(23):9818-26
pubmed: 21049934
EMBO J. 1991 Aug;10(8):2095-104
pubmed: 1648479
Mol Biol Cell. 2010 Jun 15;21(12):1982-90
pubmed: 20427572
Methods Enzymol. 2010;470:393-426
pubmed: 20946819
Mol Cell Biol. 2007 Nov;27(21):7414-24
pubmed: 17785431
FEMS Microbiol Lett. 1993 Mar 1;107(2-3):251-3
pubmed: 8472906
J Biol Chem. 1998 Dec 11;273(50):33311-9
pubmed: 9837904
Curr Genet. 1994 Oct;26(4):295-301
pubmed: 7882422
Appl Microbiol Biotechnol. 2008 Jan;77(5):1083-91
pubmed: 17955190
mBio. 2017 Dec 12;8(6):
pubmed: 29233898
Methods Enzymol. 2006;409:329-45
pubmed: 16793410
Genome Res. 2012 May;22(5):975-84
pubmed: 22399573
PLoS Genet. 2008 Jul 11;4(7):e1000123
pubmed: 18617998
Nat Commun. 2017 Oct 13;8(1):922
pubmed: 29030545
Glycobiology. 2003 Apr;13(4):17R-27R
pubmed: 12626396
Nat Protoc. 2012 Mar 01;7(3):562-78
pubmed: 22383036
Mol Microbiol. 1993 May;8(5):927-43
pubmed: 8355617
PLoS Biol. 2008 Apr 15;6(4):e83
pubmed: 18416601
EMBO J. 1995 Apr 3;14(7):1360-71
pubmed: 7729414
PLoS Pathog. 2013 Aug;9(8):e1003447
pubmed: 23966851
Gene. 1995 Apr 14;156(1):119-22
pubmed: 7737504
Curr Genet. 1995 Jan;27(2):110-22
pubmed: 7788713
PLoS Genet. 2006 Feb;2(2):e13
pubmed: 16462944
Nat Commun. 2016 Feb 19;7:10690
pubmed: 26891683
Trends Biochem Sci. 1995 Jan;20(1):3-10
pubmed: 7878741
PLoS Biol. 2010 Feb 23;8(2):e1000317
pubmed: 20186264
Proc Natl Acad Sci U S A. 2015 May 12;112(19):6122-7
pubmed: 25918381
Mol Biol Cell. 2008 Jan;19(1):352-67
pubmed: 17959824
Biochim Biophys Acta. 2013 Nov;1830(11):5204-10
pubmed: 23911748
J Bacteriol. 1992 Jun;174(12):4183-8
pubmed: 1597433
Biochim Biophys Acta. 1998 Aug 10;1366(1-2):139-49
pubmed: 9714780
Int J Mol Sci. 2009 Sep 01;10(9):3793-3810
pubmed: 19865519
Mol Cell Biol. 2004 May;24(9):3670-81
pubmed: 15082763
Proc Natl Acad Sci U S A. 1983 Mar;80(6):1730-4
pubmed: 6300872
Mol Gen Genet. 1986 Aug;204(2):310-6
pubmed: 3020369
Anal Chem. 2010 Apr 15;82(8):3212-21
pubmed: 20349993
PLoS Biol. 2012;10(5):e1001325
pubmed: 22589700
Biochem J. 2013 Sep 1;454(2):227-37
pubmed: 23763276
Bioinformatics. 2009 May 1;25(9):1105-11
pubmed: 19289445
Science. 2014 Feb 28;343(6174):1245114
pubmed: 24436182
FEMS Yeast Res. 2004 Sep;4(8):773-87
pubmed: 15450184
Proc Natl Acad Sci U S A. 2015 May 12;112(19):6116-21
pubmed: 25918382
Nature. 2002 Mar 21;416(6878):326-30
pubmed: 11907579
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Aug 15;871(2):236-42
pubmed: 18502704
FEMS Yeast Res. 2002 May;2(2):183-201
pubmed: 12702307
Mol Cell. 2004 Oct 22;16(2):293-9
pubmed: 15494315
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
Yeast. 1992 Mar;8(3):183-92
pubmed: 1315471
J Bacteriol. 1987 Nov;169(11):4941-5
pubmed: 2822656
Nat Genet. 2005 Dec;37(12):1333-40
pubmed: 16273108
Eur J Biochem. 1993 Mar 1;212(2):315-23
pubmed: 8444170
Genetics. 1989 May;122(1):19-27
pubmed: 2659436
Genes Dev. 2006 May 1;20(9):1150-61
pubmed: 16618799
Mol Gen Genet. 1995 Dec 20;249(6):655-64
pubmed: 8544831
FEMS Microbiol Rev. 1993 Apr;10(3-4):229-42
pubmed: 8318258
Genetics. 2011 Jun;188(2):325-38
pubmed: 21467572
Curr Genet. 1993;23(4):281-9
pubmed: 8467527
Eur J Biochem. 1993 Sep 15;216(3):849-61
pubmed: 8404905
Genetics. 2014 Sep;198(1):369-82
pubmed: 24970865
Mol Cell Biol. 2006 Sep;26(17):6487-501
pubmed: 16914733