IL-15 and IL-23 synergize to trigger Th17 response by CLA
Antibodies, Neutralizing
/ pharmacology
CD4-Positive T-Lymphocytes
/ metabolism
Coculture Techniques
Epidermal Cells
HLA-DR Antigens
/ metabolism
Histocompatibility Antigens Class I
/ metabolism
Humans
Interferon-gamma
/ metabolism
Interleukin-15
/ antagonists & inhibitors
Interleukin-17
/ metabolism
Interleukin-23
/ metabolism
Interleukin-23 Subunit p19
/ antagonists & inhibitors
Lymphocyte Activation
/ drug effects
Memory T Cells
/ metabolism
Oligosaccharides
/ metabolism
Primary Cell Culture
Psoriasis
/ immunology
Recombinant Proteins
/ pharmacology
Sialyl Lewis X Antigen
/ analogs & derivatives
CLA
IL-15
IL-23
Th17
psoriasis
Journal
Experimental dermatology
ISSN: 1600-0625
Titre abrégé: Exp Dermatol
Pays: Denmark
ID NLM: 9301549
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
revised:
24
04
2020
received:
02
01
2020
accepted:
04
05
2020
pubmed:
2
6
2020
medline:
16
2
2022
entrez:
2
6
2020
Statut:
ppublish
Résumé
IL-15 has emerged as a potentially relevant target in the IL-17 response in psoriasis. However, its mechanism is poorly characterized in humans. IL-15 and IL-23 are constitutively expressed in the psoriatic lesion. Also, IL-15 is considered a susceptibility-associated gene in psoriasis, as are IL-23R, and HLACW6. Here, we studied the effect of IL-15 and IL-23 stimulation on the cytokine response of CLA+/CLA- T cells from 9 psoriasis patients and 3 healthy control subjects. To this end, CLA + and CLA- T cells from blood samples were cultured with epidermal cells from skin biopsies and treated with IL-15 and IL-23. After five days of culture, cytokines in supernatant were measured by ELISA or fluorescent bead-based immunoassay. There was a statistically significant increase in IL-17F and IL-17A production (P < .001) in cocultures of psoriasis skin-homing CLA + T cells with epidermal cells when stimulated with IL-15 and IL-23, but this effect was not observed in the cells of healthy controls. Interestingly, this response was reduced by around 50 to 80% by blocking HLA class I and II molecules. Our results point to the synergic action of IL-15 and IL-23 selectively for CLA + cells in psoriasis, leading to the induction of Th17 cell-related cytokines.
Substances chimiques
6-sulfo sialyl Lewis X
0
Antibodies, Neutralizing
0
HLA-DR Antigens
0
Histocompatibility Antigens Class I
0
IL15 protein, human
0
IL17A protein, human
0
IL17F protein, human
0
IL23A protein, human
0
Interleukin-15
0
Interleukin-17
0
Interleukin-23
0
Interleukin-23 Subunit p19
0
Oligosaccharides
0
Recombinant Proteins
0
Sialyl Lewis X Antigen
0
Interferon-gamma
82115-62-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
630-638Informations de copyright
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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