Significant Short-Term Shifts in the Microbiomes of Smokers With Periodontitis After Periodontal Therapy With Amoxicillin & Metronidazole as Revealed by 16S rDNA Amplicon Next Generation Sequencing.
16S rDNA amplicon sequencing
microbiota
periodontal therapy
smoking
systemic antibiotics
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2020
2020
Historique:
received:
16
01
2020
accepted:
30
03
2020
entrez:
2
6
2020
pubmed:
2
6
2020
medline:
22
6
2021
Statut:
epublish
Résumé
The aim of this follow-up study was, to compare the effects of mechanical periodontal therapy with or without adjunctive amoxicillin and metronidazole on the subgingival microbiome of smokers with periodontitis using 16S rDNA amplicon next generation sequencing. Fifty-four periodontitis patients that smoke received either non-surgical periodontal therapy with adjunctive amoxicillin and metronidazole (n = 27) or with placebos (n = 27). Subgingival plaque samples were taken before and two months after therapy. Bacterial genomic DNA was isolated and the V4 hypervariable region of the bacterial 16S rRNA genes was amplified. Up to 96 libraries were normalized and pooled for Illumina MiSeq paired-end sequencing with almost fully overlapping 250 base pairs reads. Exact ribosomal sequence variants (RSVs) were inferred with DADA2. Microbial diversity and changes on the genus and RSV level were analyzed with non-parametric tests and a negative binomial regression model, respectively. Before therapy, the demographic, clinical, and microbial parameters were not significantly different between the placebo and antibiotic groups. Two months after the therapy, clinical parameters improved and there was a significantly increased dissimilarity of microbiomes between the two groups. In the antibiotic group, there was a significant reduction of genera classified as
Identifiants
pubmed: 32477961
doi: 10.3389/fcimb.2020.00167
pmc: PMC7232543
doi:
Substances chimiques
Anti-Bacterial Agents
0
DNA, Ribosomal
0
RNA, Ribosomal, 16S
0
Metronidazole
140QMO216E
Amoxicillin
804826J2HU
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
167Informations de copyright
Copyright © 2020 Hagenfeld, Matern, Prior, Harks, Eickholz, Lorenz, Kim, Kocher, Meyle, Kaner, Schlagenhauf, Harmsen and Ehmke.
Références
Nucleic Acids Res. 2013 Jan 7;41(1):e1
pubmed: 22933715
PLoS One. 2019 Jun 19;14(6):e0218266
pubmed: 31216300
J Clin Periodontol. 2017 Mar;44 Suppl 18:S94-S105
pubmed: 28266116
PLoS One. 2013 Apr 22;8(4):e61217
pubmed: 23630581
J Clin Periodontol. 2001 May;28(5):377-88
pubmed: 11350499
Microbiome. 2018 Dec 17;6(1):226
pubmed: 30558668
Aust Dent J. 2015 Jun;60(2):225-32
pubmed: 25283721
J Clin Periodontol. 2013 May;40(5):483-92
pubmed: 23489056
J Clin Periodontol. 1989 Feb;16(2):128-31
pubmed: 2921374
J Dent Res. 2014 Aug;93(8):725-32
pubmed: 24898946
J Periodontol. 1984 Jun;55(6):325-35
pubmed: 6376759
J Clin Periodontol. 2015 Sep;42(9):832-842
pubmed: 26250060
Int J Syst Evol Microbiol. 2000 Mar;50 Pt 2:679-688
pubmed: 10758875
J Clin Periodontol. 1998 Feb;25(2):134-44
pubmed: 9495612
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
Genome Biol. 2014;15(12):550
pubmed: 25516281
J Clin Periodontol. 2005 Feb;32(2):200-6
pubmed: 15691352
PLoS One. 2018 Apr 18;13(4):e0195534
pubmed: 29668720
Nucleic Acids Res. 2007;35(21):7188-96
pubmed: 17947321
J Clin Periodontol. 2008 Oct;35(10):885-96
pubmed: 18727657
J Periodontal Res. 2019 Aug;54(4):435-443
pubmed: 30851050
J Periodontol. 2014 Apr;85(4):581-91
pubmed: 23826648
J Dent Res. 2014 Sep;93(9):846-58
pubmed: 25074492
J Periodontol. 2001 May;72(5):666-71
pubmed: 11394403
J Clin Periodontol. 2014 Nov;41(11):1037-47
pubmed: 25139209
J Dent Res. 2010 Nov;89(11):1247-53
pubmed: 20739702
J Clin Periodontol. 2018 Jun;45 Suppl 20:S149-S161
pubmed: 29926495
Mol Oral Microbiol. 2015 Jun;30(3):227-41
pubmed: 25283067