Blocking of Immunoblots and Incubation with Antibodies: Procedure for Immunoblots Prepared with Immunoprecipitated Protein Antigens.
Journal
Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530
Informations de publication
Date de publication:
01 06 2020
01 06 2020
Historique:
entrez:
3
6
2020
pubmed:
3
6
2020
medline:
8
6
2021
Statut:
epublish
Résumé
Detection of the protein antigens in immunoblots prepared with immunoprecipitated protein antigens can be affected by the presence of high amounts of the immunoprecipitating antibodies. When it is not possible to use the immunoprecipitating antibodies and the primary antibodies raised in different species, this protocol provides a convenient and inexpensive alternative to achieve optimal detection of immunoprecipitated protein antigens. In this protocol, a nitrocellulose or polyvinylidene fluoride membrane containing immunoprecipitated protein samples is rinsed with ultrapure H
Identifiants
pubmed: 32482899
pii: 2020/6/pdb.prot098475
doi: 10.1101/pdb.prot098475
doi:
Substances chimiques
Antibodies
0
Antigens
0
Azo Compounds
0
Buffers
0
Fluorescent Dyes
0
Membranes, Artificial
0
Polyvinyls
0
Avidin
1405-69-2
polyvinylidene fluoride
24937-79-9
Biotin
6SO6U10H04
Collodion
9004-70-0
ponceau S
T1C2UGS05F
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
098475Informations de copyright
© 2020 Cold Spring Harbor Laboratory Press.