AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex.
Amino Acid Sequence
Bacteria
/ genetics
Bacterial Proteins
/ chemistry
Bacteriophages
/ genetics
CRISPR-Cas Systems
Cryoelectron Microscopy
DNA
/ chemistry
Models, Molecular
Multiprotein Complexes
/ chemistry
Nucleic Acid Conformation
Protein Binding
Protein Conformation
Proteus penneri
/ genetics
RNA, Guide, Kinetoplastida
/ chemistry
Sequence Homology, Amino Acid
Viral Proteins
/ chemistry
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
01 06 2020
01 06 2020
Historique:
received:
06
03
2020
accepted:
07
05
2020
entrez:
3
6
2020
pubmed:
3
6
2020
medline:
18
8
2020
Statut:
epublish
Résumé
Bacteria have evolved sophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection against phage infection. In turn, phages have evolved a broad spectrum of anti-CRISPRs that suppress these immune systems. Here we report structures of anti-CRISPR protein IF9 (AcrIF9) in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy). In addition to sterically blocking the hybridization of complementary dsDNA to the CRISPR RNA, our results show that AcrIF9 binding also promotes non-sequence-specific engagement with dsDNA, potentially sequestering the complex from target DNA. These findings highlight the versatility of anti-CRISPR mechanisms utilized by phages to suppress CRISPR-mediated immune systems.
Identifiants
pubmed: 32483187
doi: 10.1038/s41467-020-16512-1
pii: 10.1038/s41467-020-16512-1
pmc: PMC7264359
doi:
Substances chimiques
Bacterial Proteins
0
Multiprotein Complexes
0
RNA, Guide
0
Viral Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2730Subventions
Organisme : NIBIB NIH HHS
ID : DP2 EB020402
Pays : United States
Organisme : NIH HHS
ID : S10 OD021634
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM134867
Pays : United States
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