Coordinated Activation of ARF1 GTPases by ARF-GEF GNOM Dimers Is Essential for Vesicle Trafficking in Arabidopsis.
Arabidopsis
/ metabolism
Arabidopsis Proteins
/ metabolism
Cell Membrane
/ metabolism
DNA-Binding Proteins
/ metabolism
Guanine Nucleotide Exchange Factors
/ metabolism
Models, Biological
Phenotype
Plants, Genetically Modified
Protein Binding
Protein Multimerization
Transcription Factors
/ metabolism
Transport Vesicles
/ metabolism
Journal
The Plant cell
ISSN: 1532-298X
Titre abrégé: Plant Cell
Pays: England
ID NLM: 9208688
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
25
03
2020
revised:
19
05
2020
accepted:
30
05
2020
pubmed:
4
6
2020
medline:
7
4
2021
entrez:
4
6
2020
Statut:
ppublish
Résumé
Membrane trafficking maintains the organization of the eukaryotic cell and delivers cargo proteins to their subcellular destinations, such as sites of action or degradation. The formation of membrane vesicles requires the activation of the ADP-ribosylation factor ARF GTPase by the SEC7 domain of ARF guanine-nucleotide exchange factors (ARF-GEFs), resulting in the recruitment of coat proteins by GTP-bound ARFs. In vitro exchange assays were done with monomeric proteins, although ARF-GEFs form dimers in vivo. This feature is conserved across eukaryotes, although its biological significance is unknown. Here, we demonstrate the proximity of ARF1•GTPs in vivo by fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy, mediated through coordinated activation by dimers of Arabidopsis (
Identifiants
pubmed: 32487565
pii: tpc.20.00240
doi: 10.1105/tpc.20.00240
pmc: PMC7401013
doi:
Substances chimiques
ARF1 protein, Arabidopsis
0
Arabidopsis Proteins
0
DNA-Binding Proteins
0
GNOM protein, Arabidopsis
0
Guanine Nucleotide Exchange Factors
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2491-2507Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 American Society of Plant Biologists. All rights reserved.
Références
EMBO J. 1998 Dec 1;17(23):6903-11
pubmed: 9843496
Methods Mol Biol. 2018;1691:139-158
pubmed: 29043675
J Biol Chem. 2007 Sep 28;282(39):28834-42
pubmed: 17640864
PLoS Genet. 2018 Nov 15;14(11):e1007795
pubmed: 30439956
Nat Methods. 2014 Feb;11(2):121-2
pubmed: 24481215
Nat Rev Mol Cell Biol. 2011 Jun;12(6):362-75
pubmed: 21587297
Curr Biol. 2014 Jun 16;24(12):1383-1389
pubmed: 24881875
J Cell Biol. 2011 Sep 5;194(5):765-77
pubmed: 21893600
J Cell Biol. 1997 Dec 15;139(6):1485-93
pubmed: 9396754
Traffic. 2008 Sep;9(10):1629-52
pubmed: 18764818
Traffic. 2007 Nov;8(11):1476-85
pubmed: 17850229
J Cell Sci. 2018 Mar 13;131(5):
pubmed: 29535154
Nature. 2003 Dec 4;426(6966):525-30
pubmed: 14654833
Annu Rev Cell Dev Biol. 2007;23:579-611
pubmed: 17506703
Plant Cell. 2000 Mar;12(3):343-56
pubmed: 10715321
Methods Mol Biol. 2017;1662:159-170
pubmed: 28861826
J Biol Chem. 2011 Oct 21;286(42):36898-906
pubmed: 21828055
Cell Mol Life Sci. 2008 Nov;65(21):3433-45
pubmed: 18604628
Mol Cell. 2003 Dec;12(6):1403-11
pubmed: 14690595
Science. 1999 Oct 8;286(5438):316-8
pubmed: 10514379
Nat Plants. 2016 Mar 07;2:16017
pubmed: 27249560
Development. 2004 Jan;131(2):389-400
pubmed: 14681187
Mol Genet Genomics. 2009 Oct;282(4):329-50
pubmed: 19669794
Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):9913-8
pubmed: 10954741
Mol Gen Genet. 1996 Apr 10;250(6):681-91
pubmed: 8628228
Mol Cell. 1999 Mar;3(3):275-85
pubmed: 10198630
Cell. 2003 Jan 24;112(2):219-30
pubmed: 12553910
Protoplasma. 2014 Mar;251(2):383-94
pubmed: 24390247
Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11731-6
pubmed: 18689681
Nat Rev Mol Cell Biol. 2006 May;7(5):347-58
pubmed: 16633337
J Biol Chem. 1993 Mar 25;268(9):6560-6
pubmed: 8454626
J Biol Chem. 1994 Jan 14;269(2):1437-48
pubmed: 8288610
Plant Cell. 1990 Nov;2(11):1091-1106
pubmed: 12354950
Plant Cell. 2008 Jan;20(1):142-51
pubmed: 18203920
Semin Cell Dev Biol. 2018 Aug;80:85-93
pubmed: 29024759