The C-terminal 17 amino acids of the photoreceptor UVR8 is involved in the fine-tuning of UV-B signaling.
Journal
Journal of integrative plant biology
ISSN: 1744-7909
Titre abrégé: J Integr Plant Biol
Pays: China (Republic : 1949- )
ID NLM: 101250502
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
10
02
2020
accepted:
01
06
2020
pubmed:
4
6
2020
medline:
29
5
2021
entrez:
4
6
2020
Statut:
ppublish
Résumé
Plant UV-B responses are mediated by the photoreceptor UV RESISTANCE LOCUS 8 (UVR8). In response to UV-B irradiation, UVR8 homodimers dissociate into monomers that bind to the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). The interaction of the C27 domain in the C-terminal tail of UVR8 with the WD40 domain of COP1 is critical for UV-B signaling. However, the function of the last 17 amino acids (C17) of the C-terminus of UVR8, which are adjacent to C27, is unknown, although they are largely conserved in land plants. In this study, we established that Arabidopsis thaliana UVR8 C17 binds to full-length UVR8, but not to COP1, and reduces COP1 binding to the remaining portion of UVR8, including C27. We hypothesized that overexpression of C17 in a wild-type background would have a dominant negative effect on UVR8 activity; however, C17 overexpression caused strong silencing of endogenous UVR8, precluding a detailed analysis. We therefore generated YFP-UVR8
Substances chimiques
Amino Acids
0
Arabidopsis Proteins
0
Chromosomal Proteins, Non-Histone
0
Uvr8 protein, Arabidopsis
0
AT2G32950 protein, Arabidopsis
EC 2.3.2.27
Ubiquitin-Protein Ligases
EC 2.3.2.27
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1327-1340Subventions
Organisme : National Key Research and Development Program of China
ID : 2018YFD1000800
Organisme : National Natural Science Foundation of China
ID : 31870261
Organisme : Shanghai Pujiang Program
ID : 18PJ1404400
Organisme : Natural Science Foundation of Shanghai
ID : 18ZR1419600
Organisme : The Agri-X Interdisciplinary Fund of Shanghai Jiao Tong University
ID : Agri-X2017006
Organisme : Youngman Research at SJTU
ID : 18X100040018
Informations de copyright
© 2020 Institute of Botany, Chinese Academy of Sciences.
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