Killing of bacterial spores by dodecylamine and its effects on spore inner membrane properties.
Bacillus
antimicrobials
bacterial spores
disinfection
spores
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
02
03
2020
revised:
30
04
2020
accepted:
26
05
2020
pubmed:
4
6
2020
medline:
19
12
2020
entrez:
4
6
2020
Statut:
ppublish
Résumé
The objective of this study was to determine the effects of Ca-dipicolinic acid (CaDPA), cortex-lytic enzymes (CLEs), the inner membrane (IM) CaDPA channel and coat on spore killing by dodecylamine. Bacillus subtilis spores, wild-type, CaDPA-less due to the absence of DPA synthase or the IM CaDPA channel, or lacking CLEs, were dodecylamine-treated and spore viability and vital staining were all determined. Dodecylamine killed intact wild-type and CaDPA-less B. subtilis spores similarly, and also killed intact Clostridiodes difficile spores ± CaDPA, with up to 99% killing with 1 mol l Dodecylamine spore killing does not require CaDPA channels, CaDPA or CLEs. The lack of CaDPA in decoated spores allowed strong PI staining of the spore core, indicating loss of these spores IM permeability barrier. This work gives new information on killing bacterial spores by dodecylamine, and how spore IM's relative impermeability is maintained.
Substances chimiques
Amines
0
Anti-Bacterial Agents
0
Bacterial Proteins
0
Picolinic Acids
0
dipicolinic acid
UE81S5CQ0G
dodecylamine
YWY9OD6A2K
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1511-1522Subventions
Organisme : Tufts University
Informations de copyright
© 2020 The Society for Applied Microbiology.
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