Phosphorylation of the RecQ Helicase Sgs1/BLM Controls Its DNA Unwinding Activity during Meiosis and Mitosis.
Cdc28(CDK1)
Cdc5(PLK1)
DNA repair
Sgs1(BLM)-Top3-Rmi1
cell cycle
crossover
homologous recombination
meiosis
mitosis
noncrossover
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
22 06 2020
22 06 2020
Historique:
received:
06
12
2019
revised:
18
03
2020
accepted:
13
05
2020
pubmed:
7
6
2020
medline:
2
1
2021
entrez:
7
6
2020
Statut:
ppublish
Résumé
The Bloom's helicase ortholog, Sgs1, orchestrates the formation and disengagement of recombination intermediates to enable controlled crossing-over during meiotic and mitotic DNA repair. Whether its enzymatic activity is temporally regulated to implement formation of noncrossovers prior to the activation of crossover-nucleases is unknown. Here, we show that, akin to the Mus81-Mms4, Yen1, and MutLγ-Exo1 nucleases, Sgs1 helicase function is under cell-cycle control through the actions of CDK and Cdc5 kinases. Notably, however, whereas CDK and Cdc5 unleash nuclease function during M phase, they act in concert to stimulate Sgs1 activity during S phase/prophase I. Mechanistically, CDK-mediated phosphorylation enhances the velocity and processivity of Sgs1, which stimulates DNA unwinding in vitro and joint molecule processing in vivo. Subsequent hyper-phosphorylation by Cdc5 appears to reduce the activity of Sgs1, while activating Mus81-Mms4 and MutLγ-Exo1. These findings suggest a concerted mechanism driving orderly formation of noncrossover and crossover recombinants in meiotic and mitotic cells.
Identifiants
pubmed: 32504558
pii: S1534-5807(20)30405-6
doi: 10.1016/j.devcel.2020.05.016
pii:
doi:
Substances chimiques
Cell Cycle Proteins
0
DNA, Fungal
0
Saccharomyces cerevisiae Proteins
0
Protein Serine-Threonine Kinases
EC 2.7.11.1
CDC5 protein, S cerevisiae
EC 2.7.11.21
SGS1 protein, S cerevisiae
EC 3.6.1.-
RecQ Helicases
EC 3.6.4.12
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
706-723.e5Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.