Unlabeled lysophosphatidic acid receptor binding in free solution as determined by a compensated interferometric reader.

Biological Assay Interferometry Ligands Light Protein Binding Receptors, Lysophosphatidic Acid / metabolism
G protein-coupled receptor free-solution assay-compensated interferometric reader interferometry lipid signaling lysophospholipids molecular interaction receptor binding assay

Journal

Journal of lipid research
ISSN: 1539-7262
Titre abrégé: J Lipid Res
Pays: United States
ID NLM: 0376606

Informations de publication

Date de publication:
08 2020
Historique:
received: 03 05 2020
revised: 01 06 2020
pubmed: 10 6 2020
medline: 14 8 2021
entrez: 10 6 2020
Statut: ppublish

Résumé

Native interactions between lysophospholipids (LPs) and their cognate LP receptors are difficult to measure because of lipophilicity and/or the adhesive properties of lipids, which contribute to high levels of nonspecific binding in cell membrane preparations. Here, we report development of a free-solution assay (FSA) where label-free LPs bind to their cognate G protein-coupled receptors (GPCRs), combined with a recently reported compensated interferometric reader (CIR) to quantify native binding interactions between receptors and ligands. As a test case, the binding parameters between lysophosphatidic acid (LPA) receptor 1 (LPA

Identifiants

DOI: 10.1194/jlr.D120000880 PMID: 32513900 PMC: PMC7397748
pubmed: 32513900
pii: S0022-2275(20)43492-3
doi: 10.1194/jlr.D120000880
pmc: PMC7397748
doi:

Substances chimiques

Ligands 0
Receptors, Lysophosphatidic Acid 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

1244-1251

Subventions

Organisme : NINDS NIH HHS
ID : R01 NS084398
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS103940
Pays : United States

Informations de copyright

Copyright © 2020 Ray et al. Published by The American Society for Biochemistry and Molecular Biology, Inc.

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Auteurs

Manisha Ray (M)

Degenerative Disease Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037.

Kazufumi Nagai (K)

Degenerative Disease Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037.

Yasuyuki Kihara (Y)

Degenerative Disease Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037.

Amanda Kussrow (A)

Department of Chemistry and Vanderbilt Institute for Chemical Biology, Vanderbilt University, Nashville, TN 37235.

Michael N Kammer (MN)

Department of Chemistry and Vanderbilt Institute for Chemical Biology, Vanderbilt University, Nashville, TN 37235.

Aaron Frantz (A)

Degenerative Disease Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037.
Biomedical Sciences Graduate Program, University of California San Diego, La Jolla, CA 92037.

Darryl J Bornhop (DJ)

Department of Chemistry and Vanderbilt Institute for Chemical Biology, Vanderbilt University, Nashville, TN 37235.

Jerold Chun (J)

Degenerative Disease Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037 jchun@SBPdiscovery.org.
ORCID: 0000-0003-3964-0921

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Dosage biologique › Unlabeled lysophosphatidic acid receptor...
questionsmedicales.fr › Techniques d'investigation › Interférométrie › Unlabeled lysophosphatidic acid receptor...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Ligands › Unlabeled lysophosphatidic acid receptor...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement non ionisant › Lumière › Unlabeled lysophosphatidic acid receptor...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Unlabeled lysophosphatidic acid receptor...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs couplés aux protéines G › Récepteurs aux lysophospholipides › Récepteurs à l'acide phosphatidique › Unlabeled lysophosphatidic acid receptor...