The ever-expanding tcp conjugation locus of pCW3 from Clostridium perfringens.

Clostridium perfringens / genetics Conjugation, Genetic Plasmids / genetics Tetracycline Resistance
Adhesin Clostridium perfringens Conjugation DNA methyltransferase Plasmid Sortase Type IV secretion system (T4SS) pCW3 tcp locus

Journal

Plasmid
ISSN: 1095-9890
Titre abrégé: Plasmid
Pays: United States
ID NLM: 7802221

Informations de publication

Date de publication:
01 2021
Historique:
received: 24 03 2020
revised: 20 04 2020
accepted: 26 04 2020
pubmed: 12 6 2020
medline: 29 10 2021
entrez: 12 6 2020
Statut: ppublish

Résumé

The spore-forming, anaerobic Gram positive pathogen Clostridium perfringens encodes many of its disease-causing toxins on closely related conjugative plasmids. Studies of the tetracycline resistance plasmid pCW3 have identified many of the genes involved in conjugative transfer, which are located in the tcp conjugation locus. Upstream of this locus is an uncharacterised region (the cnaC region) that is highly conserved. This study examined the importance in pCW3 conjugation of several highly conserved proteins encoded in the cnaC region. Conjugative mating studies suggested that the SrtD, TcpN and Dam proteins were required for efficient pCW3 transfer between C. perfringens cells from the same strain background. The requirement of these proteins for conjugation was amplified in matings between C. perfringens cells of different strain backgrounds. Additionally, the putative collagen adhesin protein, CnaC, was only required for the optimal transfer of pCW3 between cells of different strain backgrounds. Based on these studies we postulate that CnaC, SrtD, TcpN and Dam are involved in enhancing the transfer frequency of pCW3. These studies have led to a significant expansion of the tcp conjugation locus, which now encompasses a 19 kb region.

Identifiants

DOI: 10.1016/j.plasmid.2020.102516 PMID: 32526229
pubmed: 32526229
pii: S0147-619X(20)30028-7
doi: 10.1016/j.plasmid.2020.102516
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

102516

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Sarah A Revitt-Mills (SA)

Infection and Immunity Program, Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, Victoria 3800, Australia. Electronic address: sarah.revitt-mills@monash.edu.

Thomas D Watts (TD)

Infection and Immunity Program, Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, Victoria 3800, Australia.

Dena Lyras (D)

Infection and Immunity Program, Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, Victoria 3800, Australia.

Vicki Adams (V)

Infection and Immunity Program, Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, Victoria 3800, Australia.

Julian I Rood (JI)

Infection and Immunity Program, Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, Victoria 3800, Australia.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bactéries à Gram positif formant des endospores › Clostridium › Clostridium perfringens › The ever-expanding tcp conjugation locus of...
questionsmedicales.fr › Phénomènes génétiques › Recombinaison génétique › Conjugaison génétique › The ever-expanding tcp conjugation locus of...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › The ever-expanding tcp conjugation locus of...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Résistance microbienne aux médicaments › Résistance bactérienne aux médicaments › Résistance à la tétracycline › The ever-expanding tcp conjugation locus of...