Natural pyrrolysine-biased translation of stop codons in mitochondrial peptides entirely coded by expanded codons.
Aminoacylation
Antitermination
DelRNAs
RNA editing
Stop suppression
tRNA hopping
Journal
Bio Systems
ISSN: 1872-8324
Titre abrégé: Biosystems
Pays: Ireland
ID NLM: 0430773
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
14
04
2020
revised:
02
06
2020
accepted:
02
06
2020
pubmed:
14
6
2020
medline:
2
7
2021
entrez:
14
6
2020
Statut:
ppublish
Résumé
During the noncanonical deletion transcription, k nucleotides are systematically skipped/deleted after each transcribed trinucleotide producing deletion-RNAs (delRNAs). Peptides matching delRNAs either result from (a) canonical translation of delRNAs; or (b) noncanonical translation of regular transcripts along expanded codons. Only along frame "0" (start site) (a) and (b) produce identical peptides. Here, mitochondrial mass spectrometry data analyses assume expanded codon/del-transcription with 3 + k (k from 0 to 12) nucleotides. Detected peptides map preferentially on previously identified delRNAs. More peptides were detected for k (1-12) when del-transcriptional and expanded codon translations start sites coincide (i.e. the 0
Identifiants
pubmed: 32534170
pii: S0303-2647(20)30079-4
doi: 10.1016/j.biosystems.2020.104180
pii:
doi:
Substances chimiques
Codon, Terminator
0
Peptide Fragments
0
RNA, Transfer
9014-25-9
pyrrolysine
H3214Y96LP
Lysine
K3Z4F929H6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104180Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.