Broad neutralization of SARS-related viruses by human monoclonal antibodies.
Adult
Aged
Angiotensin-Converting Enzyme 2
Antibodies, Monoclonal
/ immunology
Antibodies, Viral
/ immunology
Antibody Affinity
B-Lymphocyte Subsets
/ immunology
Betacoronavirus
/ immunology
Binding Sites
Broadly Neutralizing Antibodies
/ immunology
Cross Reactions
Epitopes
Female
Humans
Immunologic Memory
Male
Middle Aged
Neutralization Tests
Peptidyl-Dipeptidase A
/ chemistry
Protein Domains
Receptors, Coronavirus
Receptors, Virus
/ chemistry
Severe acute respiratory syndrome-related coronavirus
/ immunology
SARS-CoV-2
Severe Acute Respiratory Syndrome
/ immunology
Somatic Hypermutation, Immunoglobulin
Spike Glycoprotein, Coronavirus
/ chemistry
Young Adult
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
07 08 2020
07 08 2020
Historique:
received:
14
05
2020
accepted:
11
06
2020
pubmed:
17
6
2020
medline:
25
8
2020
entrez:
17
6
2020
Statut:
ppublish
Résumé
Broadly protective vaccines against known and preemergent human coronaviruses (HCoVs) are urgently needed. To gain a deeper understanding of cross-neutralizing antibody responses, we mined the memory B cell repertoire of a convalescent severe acute respiratory syndrome (SARS) donor and identified 200 SARS coronavirus 2 (SARS-CoV-2) binding antibodies that target multiple conserved sites on the spike (S) protein. A large proportion of the non-neutralizing antibodies display high levels of somatic hypermutation and cross-react with circulating HCoVs, suggesting recall of preexisting memory B cells elicited by prior HCoV infections. Several antibodies potently cross-neutralize SARS-CoV, SARS-CoV-2, and the bat SARS-like virus WIV1 by blocking receptor attachment and inducing S1 shedding. These antibodies represent promising candidates for therapeutic intervention and reveal a target for the rational design of pan-sarbecovirus vaccines.
Identifiants
pubmed: 32540900
pii: science.abc7424
doi: 10.1126/science.abc7424
pmc: PMC7299279
doi:
Substances chimiques
Antibodies, Monoclonal
0
Antibodies, Viral
0
Broadly Neutralizing Antibodies
0
Epitopes
0
Receptors, Coronavirus
0
Receptors, Virus
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Peptidyl-Dipeptidase A
EC 3.4.15.1
ACE2 protein, human
EC 3.4.17.23
Angiotensin-Converting Enzyme 2
EC 3.4.17.23
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
731-736Subventions
Organisme : NIAID NIH HHS
ID : R01 AI132317
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI073148
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI127521
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007288
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI142777
Pays : United States
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
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