GDSL lipase occluded stomatal pore 1 is required for wax biosynthesis and stomatal cuticular ledge formation.
OSP1
Arabidopsis
drought tolerance
epidermal permeability
stomatal outer cuticular ledge
thioesterase activity
water loss
wax biosynthesis
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
03
03
2020
accepted:
28
05
2020
pubmed:
17
6
2020
medline:
15
5
2021
entrez:
17
6
2020
Statut:
ppublish
Résumé
The plant leaf surface is coated with a waterproof cuticle layer. Cuticle facing the stomatal pore surface needs to be sculpted to form outer cuticular ledge (OCL) after stomatal maturation for efficient gas exchange. Here, we characterized the roles of Arabidopsis GDSL lipase, Occlusion of Stomatal Pore 1 (OSP1), in wax biosynthesis and stomatal OCL formation. OSP1 mutation results in significant reduction in leaf wax synthesis and occlusion of stomata, leading to increased epidermal permeability, decreased transpiration rate, and enhanced drought tolerance. We demonstrated that OSP1 activity is critical for its role in wax biosynthesis and stomatal function. In vitro enzymatic assays demonstrated that OSP1 possesses thioesterase activity, particularly on C22:0 and C26:0 acyl-CoAs. Genetic interaction analyses with CER1 (ECERIFERUM 1), CER3 (ECERIFERUM 3) and MAH1 (Mid-chain Alkane Hydroxylase 1) in wax biosynthesis and stomatal OCL formation showed that OSP1 may act upstream of CER3 in wax biosynthesis, and implicate that wax composition percentage changes and keeping ketones in a lower level play roles, at least partially, in forming stomatal ledges. Our findings provided insights into the molecular mechanism mediating wax biosynthesis and highlighted the link between wax biosynthesis and the process of stomatal OCL formation.
Substances chimiques
Arabidopsis Proteins
0
Waxes
0
Lipase
EC 3.1.1.3
CER3 protein, Arabidopsis
EC 4.1.-
Carbon-Carbon Lyases
EC 4.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1880-1896Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 The Authors New Phytologist © 2020 New Phytologist Foundation.
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