The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
19 06 2020
19 06 2020
Historique:
received:
30
12
2019
accepted:
18
05
2020
entrez:
21
6
2020
pubmed:
21
6
2020
medline:
29
8
2020
Statut:
epublish
Résumé
While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of sophisticated bioinformatic tools and field-specific biological expertise for data interpretation. Here we present the initiation of the Archaeal Proteome Project (ArcPP), a community-based effort to comprehensively analyze archaeal proteomes. Starting with the model archaeon Haloferax volcanii, we reanalyze MS datasets from various strains and culture conditions. Optimized peptide spectrum matching, with strict control of false discovery rates, facilitates identifying > 72% of the reference proteome, with a median protein sequence coverage of 51%. These analyses, together with expert knowledge in diverse aspects of cell biology, provide meaningful insights into processes such as N-terminal protein maturation, N-glycosylation, and metabolism. Altogether, ArcPP serves as an invaluable blueprint for comprehensive prokaryotic proteomics.
Identifiants
pubmed: 32561711
doi: 10.1038/s41467-020-16784-7
pii: 10.1038/s41467-020-16784-7
pmc: PMC7305310
doi:
Substances chimiques
Archaeal Proteins
0
Proteome
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3145Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM057498
Pays : United States
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