MYB4 transcription factor, a member of R2R3-subfamily of MYB domain protein, regulates cadmium tolerance via enhanced protection against oxidative damage and increases expression of PCS1 and MT1C in Arabidopsis.
Aminoacyltransferases
/ metabolism
Arabidopsis
/ metabolism
Arabidopsis Proteins
/ metabolism
Cadmium
/ metabolism
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Plant
/ physiology
Immunoblotting
Lipid Peroxidation
Metallothionein
/ metabolism
Oxidative Stress
/ drug effects
Reactive Oxygen Species
/ metabolism
Repressor Proteins
/ metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors
/ metabolism
Arabidopsis
Cadmium stress
Glutathione
MYB4 transcription factor
Oxidative stress
Phytochelatins
Journal
Plant science : an international journal of experimental plant biology
ISSN: 1873-2259
Titre abrégé: Plant Sci
Pays: Ireland
ID NLM: 9882015
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
02
02
2020
revised:
08
04
2020
accepted:
10
04
2020
entrez:
22
6
2020
pubmed:
22
6
2020
medline:
28
1
2021
Statut:
ppublish
Résumé
Here, we describe functional characterization of Arabidopsis thaliana MYB4 transcription factor, a member of R2R3-subfamily of MYB domain protein, in the regulation of Cd-stress tolerance in Arabidopsis. Transgenic Arabidopsis plants overexpressing MYB4 showed appreciable Cd tolerance than wild-type plants, while MYB4 loss of function mutant lines (atmyb4) showed increased sensitivity to Cd-stress. MYB4 overexpression lines showed strong activation of anti-oxidant defense components and increased Cd accumulation than wild-type and atmyb4 mutant lines under Cd-stress. MYB4 overexpression resulted in the coordinated activation of the expression of phytochelatin (PC) synthesis related genes and specifically enhanced the transcript abundance of phytochelatin synthase 1 (PCS1) and metallothionein 1C (MT1C) genes under Cd-stress. In contrast, atmyb4 mutant lines showed reduced Cd accumulation and compromised expression of PC-synthesis related genes. Electrophoretic gel mobility shift assays have demonstrated specific binding activity of recombinant AtMYB4 to the putative MYB4-binding motifs ACCAACCAA and GGTAGGT identified in the promoters of PCS1 and MT1C genes, respectively. Further analyses have revealed that MYB4 binds directly to PCS1 and MT1C promoters in vivo and positively regulates their transcriptional expression, suggesting that PCS1 and MT1C are the key targets of MYB4. Overall, our results have provided evidence that MYB4 regulates Cd-tolerance via the coordinated activity of improved anti-oxidant defense system and through the enhanced expression of PCS1 and MT1C under Cd-stress in Arabidopsis.
Identifiants
pubmed: 32563471
pii: S0168-9452(20)30104-7
doi: 10.1016/j.plantsci.2020.110501
pii:
doi:
Substances chimiques
Arabidopsis Proteins
0
MT1c protein, Arabidopsis
0
Myb4 protein, Arabidopsis
0
Reactive Oxygen Species
0
Repressor Proteins
0
Transcription Factors
0
Cadmium
00BH33GNGH
Metallothionein
9038-94-2
Aminoacyltransferases
EC 2.3.2.-
AT5G44070 protein, Arabidopsis
EC 2.3.2.15
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110501Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The Authors declare that there is no conflict of interest.