Application of a non-invasive oral brushing procedure based on bisulfite sequencing of a 13-gene panel to study high-risk OSCC patients.
Adult
Aged
Biomarkers, Tumor
/ genetics
Biopsy
/ methods
DNA Methylation
Diagnosis, Differential
Early Detection of Cancer
/ methods
Epigenesis, Genetic
Epithelial Cells
/ pathology
Feasibility Studies
Female
Gene Expression Regulation, Neoplastic
Healthy Volunteers
Humans
Leukoplakia, Oral
/ diagnosis
Lichen Planus, Oral
/ diagnosis
Male
Middle Aged
Mouth Mucosa
/ cytology
Mouth Neoplasms
/ diagnosis
Prospective Studies
Sequence Analysis, DNA
Specimen Handling
/ methods
Squamous Cell Carcinoma of Head and Neck
/ genetics
Bisulfite sequencing
algorithm
oral brushing
oral carcinoma
oral leukoplakia
oral lichen planus
quantitative DNA methylation analysis
Journal
Cancer biomarkers : section A of Disease markers
ISSN: 1875-8592
Titre abrégé: Cancer Biomark
Pays: Netherlands
ID NLM: 101256509
Informations de publication
Date de publication:
2020
2020
Historique:
pubmed:
23
6
2020
medline:
15
4
2021
entrez:
23
6
2020
Statut:
ppublish
Résumé
A non-invasive sampling procedure for the early detection of Oral Squamous Cell Carcinoma (OSCC) based on DNA methylation analysis of a panel of 13 genes was applied in 4 different OSCC risk-group of patients. Aim of the study is to evaluate the between-group differences and the variables related to the methylation profile of each group. Oral brushing samples were collected from 54 healthy subjects, 31 Oral Leukoplakia (OL) patients, 18 Oral Lichen Planus (OLP) patients and 26 patients previously treated for OSCC. Each sample was considered positive or negative in relation to a predefined cut-off value. None of the samples from 54 healthy subjects were positive, whereas 22/31 OL, 3/18 OLP and 8/26 surgically treated OSCC samples showed positive values with respect to the cut-off. In OL patients, dysplasia was the only variable significantly related to positive values: 10/10 OLs with high-grade dysplasia were positive with respect to 12/21 OLs without dysplasia (Chi 6.039, p< 0.05). DNA methylation analysis in epithelial cells collected by oral brushing seems to be a promising genetic method to distinguish lesions at high risk of developing OSCC. Larger population studies and an adequate follow-up period are necessary to confirm these preliminary data.
Sections du résumé
BACKGROUND
BACKGROUND
A non-invasive sampling procedure for the early detection of Oral Squamous Cell Carcinoma (OSCC) based on DNA methylation analysis of a panel of 13 genes was applied in 4 different OSCC risk-group of patients. Aim of the study is to evaluate the between-group differences and the variables related to the methylation profile of each group.
METHODS
METHODS
Oral brushing samples were collected from 54 healthy subjects, 31 Oral Leukoplakia (OL) patients, 18 Oral Lichen Planus (OLP) patients and 26 patients previously treated for OSCC. Each sample was considered positive or negative in relation to a predefined cut-off value.
RESULTS
RESULTS
None of the samples from 54 healthy subjects were positive, whereas 22/31 OL, 3/18 OLP and 8/26 surgically treated OSCC samples showed positive values with respect to the cut-off. In OL patients, dysplasia was the only variable significantly related to positive values: 10/10 OLs with high-grade dysplasia were positive with respect to 12/21 OLs without dysplasia (Chi 6.039, p< 0.05).
CONCLUSION
CONCLUSIONS
DNA methylation analysis in epithelial cells collected by oral brushing seems to be a promising genetic method to distinguish lesions at high risk of developing OSCC. Larger population studies and an adequate follow-up period are necessary to confirm these preliminary data.
Identifiants
pubmed: 32568174
pii: CBM190422
doi: 10.3233/CBM-190422
doi:
Substances chimiques
Biomarkers, Tumor
0
Types de publication
Journal Article
Observational Study
Langues
eng
Sous-ensembles de citation
IM