Analysis of HBV Genomes Integrated into the Genomes of Human Hepatoma PLC/PRF/5 Cells by HBV Sequence Capture-Based Next-Generation Sequencing.
Carcinogenesis
/ genetics
Carcinoma, Hepatocellular
/ genetics
DNA, Viral
/ genetics
Genome, Human
/ genetics
Genome, Viral
/ genetics
Hepatitis B
/ genetics
Hepatitis B Surface Antigens
/ genetics
Hepatitis B virus
/ genetics
High-Throughput Nucleotide Sequencing
Humans
Liver Neoplasms
/ genetics
Virus Integration
/ genetics
HBV
HCC
Huh7
PLC/PRF/5
chromosome
hepatocarcinogenesis
integration
next-generation sequencing
transfection
Journal
Genes
ISSN: 2073-4425
Titre abrégé: Genes (Basel)
Pays: Switzerland
ID NLM: 101551097
Informations de publication
Date de publication:
18 06 2020
18 06 2020
Historique:
received:
25
05
2020
revised:
11
06
2020
accepted:
16
06
2020
entrez:
24
6
2020
pubmed:
24
6
2020
medline:
19
3
2021
Statut:
epublish
Résumé
Hepatitis B virus (HBV) infection is a leading cause of hepatocellular carcinoma (HCC) worldwide. The integration of HBV genomic DNA into the host genome occurs randomly, early after infection, and is associated with hepatocarcinogenesis in HBV-infected patients. Therefore, it is important to analyze HBV genome integration. We analyzed HBV genome integration in human hepatoma PLC/PRF/5 cells by HBV sequence capture-based next-generation sequencing (NGS) methods. We confirmed the results by using Sanger sequencing methods. We observed that HBV genotype A is integrated into the genome of PLC/PRF/5 cells. HBV sequence capture-based NGS is useful for the analysis of HBV genome integrants and their locations in the human genome. Among the HBV genome integrants, we performed functional analysis and demonstrated the automatic expression of some HBV proteins encoded by HBV integrants from chromosomes 3 and 11 in Huh7 cells transfected with these DNA sequences. HBV sequence capture-based NGS may be a useful tool for the assessment of HBV genome integration into the human genome in clinical samples and suggests new strategies for hepatocarcinogenesis in HBV infection.
Identifiants
pubmed: 32570699
pii: genes11060661
doi: 10.3390/genes11060661
pmc: PMC7348787
pii:
doi:
Substances chimiques
DNA, Viral
0
Hepatitis B Surface Antigens
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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